Target design and verification of insecticidal antibody against Plutella xylostella
To generate new insecticidal proteins against Plutella xylostella by mimicking Cry toxins,two genetically engineered antibodies(GEAb)were designed by using previously obtained anti-Cry1Ab idiotypic-antibody as template,main-ly by three-dimensional structure modeling and molecular docking technology.The insecticidal GEAb-GGCC of GEAb was found to show high affinity to P.xylostella brush border membrane vesicles(BBMV),and had superposed BBMV binding sites with Cry1A and Cry1B.Identification results of BBMV immunoprecipitation analysis showed that the midgut proteins bonded with GEAb-GGCC included aminopeptidas-esN(APN),B subunit of V-ATPase and polycalin.Be-cause the GEAb-GGCC is lack of α-helix structure which is responsible for pore forming in Cry1A proteins,it is specu-lated that GEAb-GGCC may activate the downstream signal pathway by binding with the midgut receptors to cause mid-gut injury,which leads to the death of P.xylostella larva.
万方数据
维普
