Development of SSR molecular markers and genetic diversity analysis of Oenanthe javanica
Dropwort is a perennial herbaceous plant of Oenanthe genus in the Apiaceae family,which is an important vegetable crop for both food and medicine.In China,the planting area of dropwort is very extensive.However,there are few studies on the identification and cultivation of its germplasm resources and the genetic information.In this study,we used the genome of Liyang Baiqin to develop simple sequence repeat(SSR)molecular markers for dropwort,and analyzed the genetic diversity of 55 samples.The phylogenetic tree was constructed by unweighted pair group method with arithmetic means(UPG-MA),and the DNA fingerprint was constructed with SSR amplified band data.The results showed that a total of 325 699 SSR loci were identified.The frequencies of single nucleotide SSR repeat units,dinucleotide SSR repeat units,trinucleotide SSR repeat units,tetranucleotide SSR repeat units,pentanucleotide SSR repeat units and hexanucleotide SSR repeat units were 33.94%,54.62%,9.31%,1.66%,0.17%and 0.29%,respectively.Among them,the number of dinucleotide SSR repeat units was the largest(177 887),and A/T(29.98%)and AT/AT(35.70%)were the more abundant repeat types.The results of UPGMA analysis in-dicated that 55 Oenanthe javanica materials could be divided into four groups by 33 pairs of highly polymorphic primers(poly-morphic information content>0.25).Four pairs of primers(Oj-084,Oj-110,Oj-112,Oj-156)were used to completely dis-tinguish 55 Oenanthe javanica materials,and the fingerprint could be constructed.The results can provide a strong basis for the identification,protection and molecular genetic breeding of Oenanthe javanica germplasm resources.
万方数据
维普
