Identification of SPL transcription factor gene family members and their response to cold stress in passion fruit
SPL(SQUAMOSA promoter-binding protein-like)is a plant-specific transcription factor that participates in many processes of plant growth and development.In order to clarify the characteristics of SPL transcription factor family members and their response to low temperature stress in passion fruit,bioinformatics methods and genome data of passion fruit were used to analyze the structure,chromosome distribution,collinear relationship of SPL family genes in passion fruit,as well as the physicochemical properties and evolutionary relationship of SPL family genes encoded proteins.The rel-ative expression differences of SPL family genes in the leaves of passion fruit seedlings were clarified by low temperature treatment and normal temperature treatment.The results showed that 19 SPL family members were identified from the ge-nome of passion fruit,which were distributed on seven chromosomes,including three pairs of collinear gene pairs.SPL gene family of passion fruit was divided into nine branches according to results of phylogenetic tree.There were many cis-acting elements in the promoter region of PeSPL genes of passion fruit,which responsed to hormones and stress.Under low temperature stress,the relative ex-pression levels of PeSPL2,PeSPL3,PeSPL6,PeSPL7,PeSPL8 and PeSPL9 were extremely significantly in-creased,and the relative expression levels of PeSPL13 and PeSPL18 were significantly decreased.The results of this study can provide a basis for further revealing the response mechanism of passion fruit SPL gene to low temperature stress.
万方数据
维普
