Effects of soaking seeds with glucosinolates on seed germination and physi-ological characteristics of broccoli
In this study,broccoli seeds were used as experimental materials,and germination experiments were car-ried out using culture dishes.Three culture conditions of distilled water,200 mmol/L NaCl and 15％PEG-6000 were set up.Six different concentrations of glucosinolates were set as 0 μg/mL(CK),0.94 μg/mL,1.88 μg/mL,3.75 μg/mL,7.50 μg/mL and 15.00 µg/mL.The effects of different mass concentrations of glucosinolates on seed germination indices,seedling morphological indices and physiological indices of broccoli were studied.The results showed that compared with CK,soaking seeds with glucosinolates had no significant effect on the germination rate and germination potential of broccoli under distilled water culture,but the root-shoot ratio of broccoli in the treatment of soaking seeds with 7.50μg/mL glucosinolates was significantly increased.The POD activity of broccoli in the treatment of soaking seeds with 1.88-7.50 μg/mL glucosinolates was significantly higher than that in the control,and the SOD and CAT ac-tivities of broccoli in the treatment of soaking seeds with 3.75 µg/mL glucosinolates were significantly increased,and the MDA content was significantly lower than that in the control.Under salt stress,soaking seeds with 7.50 µg/mL glucosino-lates could significantly increase the activities of SOD and POD,and significantly reduce the content of MDA in broccoli.Under drought stress,soaking seeds with glucosinolates could significantly improve the germination indices of broccoli seeds and significantly reduce the MDA content of broccoli.Soaking seeds with 0.94 µg/mL glucosinolates could significantly in-crease the main root length of broccoli,and soaking seeds with 1.88 μg/mL and 3.75 μg/mL glucosinolates could signifi-cantly increase the root-shoot ratio of broccoli.Soaking seeds with 15.00 μg/mL glucosinolates could significantly increase the POD activity of broccoli.The results of membership function analysis showed that the membership function values of 3.75 μg/mL glucosinolate soaking treatment under distilled water culture and drought stress and 7.50 μg/mL glucosinolate soaking treatment under salt stress were the highest.In summary,soaking seeds with glucosinolates can effectively alleviate the drought and salt damage of broccoli during seed germination and seedling stage,the optimum concentration of glucosino-lates for soaking seeds under drought stress is 3.75 µg/mL,and the optimum concentration of glucosinolates for soaking seeds under salt stress is 7.50 μg/mL.
万方数据
维普
