Optimization of macro-elements composition in the multiplication medium of Paeonia×lemoinei'Yellow Crown'based on response surface methodology
Low multiplication coefficient has been one of the obstacles limiting the industrial application of tree peony(Paeonia sect.Moutan)micropropagation technology,but most of the current studies have been focused on the use and com-bination of plant growth regulators,and less attention has been paid to the effect of medium formulation on the multiplication of test-tube seedlings of tree peony.In this study,we used Paeonia×lemoinei'Yellow Crown'as the experimental material and designed an optimization test by using Design-Expert response surface method to investigate the effects of five macro-ele-ments,such as K2SO4,MgSO4·7H2O,KH2PO4,NH4NO3,Ca(NO3)2·4H2O in WPM medium on the multiplication effect of the test-tube seedlings of Paeonia sect.Moutan,and developed a mathematical model to pre-dict the best optimized medium formulation.Results of sin-gle factor effect analysis showed that,KH2 PO4,NH4 NO3 and Ca(NO3)2·4H2O had significant or highly significant effects on the multiplication effect of the test-tube seedlings,while MgSO4·7H2O and K2SO4had no significant effects.Re-sults of interaction analysis showed that,the interaction between K2SO4 and KH2PO4 had significant or highly significant effects on plant height,and stem length,the interactions between K2SO4 and MgSO4·7H2O,KH2PO4 and NH4NO3 had sig-nificant or hightly significant effects on stem length.Based on the experimental data,the model analysis results predicted that the optimized formulations for five macro-elements were K2SO4 2 257 mg/L,MgSO4·7H2O 907 mg/L,KH2PO4 481 mg/L,NH4NO3 200 mg/L and Ca(NO3)2·4H2O 1 963 mg/L.The validation test was conducted,and the multiplication coeffi-cient,plant height,stem length and leaf number of the test-tube seedlings were 2.43,4.62 cm,1.30 cm and 6.75 respective-ly,which were slightly higher than the predicted values obtained by Design-Expert experiment and were significantly better than the control group,indicating the special multiplication medium for P.×lemoinei'Yellow Crown'was constructed suc-cessfully.The results of this study can not only greatly improve the multiplication efficiency of P.×lemoinei'Yellow Crown',but can also provide an important technical reference for the construction and improvement of multiplication culture system of other tree peony species.
tree peonymicropropagationresponse surface methodmultiplicationWPM medium
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