摘要
增殖系数低一直是制约牡丹微繁殖技术推广应用的障碍之一,但目前的研究多集中于植物生长调节剂的使用与组合,较少关注培养基配方对牡丹试管苗增殖效果的影响.本研究以皇冠牡丹(Paeonia×lemoinei'Yellow Crown')为试验材料,使用Design-Expert响应面法设计优化试验,探究WPM中5种大量元素[K2SO4、MgSO4·7H2O、KH2PO4、NH4NO3、Ca(NO3)2·4H2O]对皇冠牡丹试管苗增殖效果的影响,并建立数学模型分析预测最佳优化培养基配方.单因素效应分析结果表明,KH2PO4、NH4NO3与Ca(NO3)2·4H2O对牡丹试管苗的增殖效果有显著或极显著影响,而MgSO4·7H2O与K2SO4对牡丹试管苗的增殖效果无明显影响.交互作用分析结果表明,K2SO4与KH2PO4的交互作用对株高、茎长有显著或极显著影响,K2SO4与MgSO4·7H2O、KH2PO4与NH4NO3的交互作用对茎长有显著或极显著影响.根据试验数据构建模型进行分析预测,结果显示,5种大量元素优化配方为:K2SO4 2 257 mg/L、MgSO4·7H2O 907 mg/L、KH2PO4 481 mg/L、NH4NO3 200 mg/L与Ca(NO3)2·4H2O 1 963 mg/L.在该条件下开展验证试验,获得试管苗的增殖系数、株高、茎长与叶片数分别为2.43、4.62 cm、1.30 cm、6.75张,略高于Design-Expert试验得出的预测值,显著优于对照组,说明本研究成功优化了皇冠牡丹的专用增殖培养基配方.研究结果不仅能大幅提高皇冠牡丹的增殖效率,也为其他牡丹品种增殖培养体系的构建与改良提供重要的技术参考.
Abstract
Low multiplication coefficient has been one of the obstacles limiting the industrial application of tree peony(Paeonia sect.Moutan)micropropagation technology,but most of the current studies have been focused on the use and com-bination of plant growth regulators,and less attention has been paid to the effect of medium formulation on the multiplication of test-tube seedlings of tree peony.In this study,we used Paeonia×lemoinei'Yellow Crown'as the experimental material and designed an optimization test by using Design-Expert response surface method to investigate the effects of five macro-ele-ments,such as K2SO4,MgSO4·7H2O,KH2PO4,NH4NO3,Ca(NO3)2·4H2O in WPM medium on the multiplication effect of the test-tube seedlings of Paeonia sect.Moutan,and developed a mathematical model to pre-dict the best optimized medium formulation.Results of sin-gle factor effect analysis showed that,KH2 PO4,NH4 NO3 and Ca(NO3)2·4H2O had significant or highly significant effects on the multiplication effect of the test-tube seedlings,while MgSO4·7H2O and K2SO4had no significant effects.Re-sults of interaction analysis showed that,the interaction between K2SO4 and KH2PO4 had significant or highly significant effects on plant height,and stem length,the interactions between K2SO4 and MgSO4·7H2O,KH2PO4 and NH4NO3 had sig-nificant or hightly significant effects on stem length.Based on the experimental data,the model analysis results predicted that the optimized formulations for five macro-elements were K2SO4 2 257 mg/L,MgSO4·7H2O 907 mg/L,KH2PO4 481 mg/L,NH4NO3 200 mg/L and Ca(NO3)2·4H2O 1 963 mg/L.The validation test was conducted,and the multiplication coeffi-cient,plant height,stem length and leaf number of the test-tube seedlings were 2.43,4.62 cm,1.30 cm and 6.75 respective-ly,which were slightly higher than the predicted values obtained by Design-Expert experiment and were significantly better than the control group,indicating the special multiplication medium for P.×lemoinei'Yellow Crown'was constructed suc-cessfully.The results of this study can not only greatly improve the multiplication efficiency of P.×lemoinei'Yellow Crown',but can also provide an important technical reference for the construction and improvement of multiplication culture system of other tree peony species.
基金项目
国家自然科学基金项目(32001359)
南京林业大学大学生创新训练计划项目(2022NFUSPITP0436)
万方数据