Mining of genes related to Triadica sebifera leaf color based on transcrip-tome sequencing
Triadica sebifera is a frequently-used color-leafed plant in landscaping because of its strong salt-tolerance and remarkable leaf color change with seasons,and it has the best ornamental properties in autumn.In order to reveal the molecular mechanism of leaf coloration of Triadica sebifera,the transcriptomes of leaves of three self-selected varieties of Triadica sebifera(Lianjiu No.1,Yuntaihongjiu and Yuntaijinjiu)were sequenced before and after leaf color changed.A to-tal of 123.78 GB sequencing data were obtained,with 70 815 high-quality Unigenes,of which 48 367 were functionally an-notated.A total of 2 674 shared differentially expressed genes(DEGs)of different comparison groups were identified from the transcriptome data of three Triadica sebifera varieties before and after color transformation,through pairwise comparison.Go analysis revealed that DEGs were mainly related to biological functions such as Cell process,Cell and Binding.Through functional analysis of KEGG Pathway,53 DEGs related to leaf color were screened,including 14 genes related to anthocyanin synthesis,22 genes related to porphyrin and chlorophyll metabolism and 17 genes related to biosynthe-sis of carotenoid.The relative expression levels of genes re-lated to anthocyanin biosynthesis pathway were increased and those related to chlorophyll and carotenoid biosynthesis pathways were decreased,the relative expression levels of genes related to chlorophyll and carotenoid degradation pathways were increased,which results were consistent with the change trends of pigment content in leaves.The results of this study can provide a theoretical basis for exploring the mechanism of leaf coloration of Triadica sebifera.
Triadica sebiferaleaf color changetranscriptomegene miningmolecular mechanismcoloration
万方数据
维普
