Effects of exogenous spermidine on nitrogen metabolism,dynamic changes of endogenous hormones in leaves and fruit quality of wine grape
To investigate the effects of spermidine(Spd)on nitrogen metabolism,endogenous hormones of wine grape leaves and fruit quality,and screen out the suitable Spd treatment method for wine grape production,10-year-old Riesling grapes were used as experimental materials in this study.Spd solutions of 0.3 mmol/L(T1),0.6 mmol/L(T2),0.9 mmol/L(T3)and 1.2 mmol/L(T4)were sprayed on the leaves at flowering stage,fruit setting stage,expansion stage and veraison stage,and water was sprayed as control(CK).The nitrogen metabolism and the contents of endogenous hor-mones in leaves at different stages and the related indexes of fruit quality were measured,and the fruit quality was comprehensively evaluated by principal component analy-sis.The results showed that the appropriate concentration of exogenous Spd treatment could enhance the nitrogen me-tabolism capacity of grape leaves,increase the contents of kinetin(KT),auxin(IAA)and gibberellin(GA3)in leaves,reduce the content of abscisic acid(ABA),and improve fruit quality.Specifically,T3 treatment significantly increased the contents of nitrate nitrogen(NO3--N)and ammonium ni-trogen(NH4+-N)in leaves during the color-changing stage,which were 37.84％and 21.52％higher than those of CK,re-spectively.The contents of KT,IAA,and GA3 in leaves in T3 treatment were significantly higher than those in CK during flowering,color-changing and ripening stages.Compared with CK,T3 treatment significantly increased the contents of solu-ble solids,soluble sugars,vitamin C,glucose,sucrose,oxalic acid,tartaric acid,citric acid,malic acid,and fumaric acid in fruits.In conclusion,the appropriate concentration of Spd treatment could enhance the nitrogen metabolism capacity of Riesling grape leaves,increase the contents of KT,IAA and GA3 in leaves,reduce ABA content,and improve fruit quality.According to the results of principal component analysis,the treatment of 0.9 mmol/L Spd had the best effect.
万方数据
维普
