Difference analysis of metabolites in kiwifruit treated with different plant growth regulators based on UPLC-MS/MS
Using Jinmi kiwifruit as the test material,the effects of forchlorfenuron(CPPU)and gibberellin(GA3)on sec-ondary metabolites in fruits were studied.A total of 411 metabolites,including amino acids and their derivatives,sugars and alcohols,nucleotides and their derivatives,were detected by ultraperformance liquid chromatography tandem mass spectrometry(UPLC-MS/MS).The number of down-regulated metabolites was higher than that of up-regulated metabolites in fruits treated with 5 mg/L CPPU or 10 mg/L CPPU.The number of up-regulated metabolites was higher than that of down-regulated metabolites in fruits treated with 50 mg/L GA3 or 10 mg/L CPPU+50 mg/L GA3.Compared with the control,the single fruit weight,transverse diameter and lateral diameter of 5 mg/L CPPU treatment,10 mg/L CPPU treatment,50 mg/L GA3 treatment and 10 mg/L CPPU+50 mg/L GA3 treatment increased significantly(P＜0.05).The single fruit weight of 50 mg/L GA3 treatment,10 mg/L CPPU+50 mg/L GA3 treatment was significantly lower than that of 5 mg/L CPPU treatment and 10 mg/L CPPU treatment(P＜0.05).It can be concluded that the treatment of 10 mg/L CPPU+50 mg/L GA3 resulted in better yield and quality of Jinmi kiwifruit.This study result can provide reference for Jinmi kiwifruit cultivation and quality evaluation.
万方数据
维普
