江苏农业学报2024,Vol.40Issue(8) :1542-1551.DOI:10.3969/j.issn.1000-4440.2024.08.018

基于UPLC-MS/MS的不同植物生长调节剂处理的猕猴桃果实中代谢物差异分析

Difference analysis of metabolites in kiwifruit treated with different plant growth regulators based on UPLC-MS/MS

王前菊 王宇 仲伟敏 徐特 唐冬梅
江苏农业学报2024,Vol.40Issue(8) :1542-1551.DOI:10.3969/j.issn.1000-4440.2024.08.018

基于UPLC-MS/MS的不同植物生长调节剂处理的猕猴桃果实中代谢物差异分析

Difference analysis of metabolites in kiwifruit treated with different plant growth regulators based on UPLC-MS/MS

王前菊 1王宇 1仲伟敏 1徐特 1唐冬梅1
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作者信息

  • 1. 贵州省果树科学研究所,贵州贵阳 550006
  • 折叠

摘要

本研究以金猕为试验材料,探究氯吡脲(CPPU)和赤霉素(GA3)对果实中次生代谢物的影响.基于超高效液相色谱-串联质谱技术(UPLC-MS/MS)对代谢组进行分析,共检测到代谢物411种,包括氨基酸及其衍生物、糖及醇类、核苷酸及其衍生物等.5 mg/L CPPU处理、10 mg/L CPPU处理果实中水平下调代谢物数量高于水平上调代谢物数量,而50 mg/L GA3处理、10 mg/L CPPU+50 mg/L GA3处理果实中水平上调代谢物数量高于水平下调代谢物数量.与对照相比,5 mg/L CPPU 处理、10 mg/L CPPU 处理、50 mg/L GA3处理、10 mg/L CPPU+50 mg/L GA3处理果实单果重、横径、侧径显著提高(P<0.05).50 mg/L GA3处理、10 mg/L CPPU+50 mg/L GA3处理果实单果重显著低于5 mg/L CPPU处理、10 mg/L CPPU处理(P<0.05).综上所述,10 mg/L CPPU+50 mg/L GA3处理金猕产量和品质相对更佳,本研究结果可为金猕栽培及品质评价提供参考.

Abstract

Using Jinmi kiwifruit as the test material,the effects of forchlorfenuron(CPPU)and gibberellin(GA3)on sec-ondary metabolites in fruits were studied.A total of 411 metabolites,including amino acids and their derivatives,sugars and alcohols,nucleotides and their derivatives,were detected by ultraperformance liquid chromatography tandem mass spectrometry(UPLC-MS/MS).The number of down-regulated metabolites was higher than that of up-regulated metabolites in fruits treated with 5 mg/L CPPU or 10 mg/L CPPU.The number of up-regulated metabolites was higher than that of down-regulated metabolites in fruits treated with 50 mg/L GA3 or 10 mg/L CPPU+50 mg/L GA3.Compared with the control,the single fruit weight,transverse diameter and lateral diameter of 5 mg/L CPPU treatment,10 mg/L CPPU treatment,50 mg/L GA3 treatment and 10 mg/L CPPU+50 mg/L GA3 treatment increased significantly(P<0.05).The single fruit weight of 50 mg/L GA3 treatment,10 mg/L CPPU+50 mg/L GA3 treatment was significantly lower than that of 5 mg/L CPPU treatment and 10 mg/L CPPU treatment(P<0.05).It can be concluded that the treatment of 10 mg/L CPPU+50 mg/L GA3 resulted in better yield and quality of Jinmi kiwifruit.This study result can provide reference for Jinmi kiwifruit cultivation and quality evaluation.

关键词

猕猴桃/UPLC-MS/MS/氯吡脲(CPPU)/赤霉素(GA3)/代谢物/品质

Key words

kiwifruit/UPLC-MS/MS/forchlorfenu-ron(CPPU)/gibberellin(GA3)/metabolites/quality

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基金项目

国家柑橘产业体系猕猴桃贵阳综合试验站资助项目(CARS-26-65)

贵州省科研机构创新能力建设专项(黔科合服企20218号)

出版年

2024
江苏农业学报
江苏省农业科学院

江苏农业学报

CSTPCD北大核心
影响因子:1.093
ISSN:1000-4440
参考文献量21
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