Rice rolled-leaf mutants are important resources for studying the mechanisms of leaf development and breed-ing desirable plant varieties.In this study,a rolled-leaf mutant(named rl-z)with stable inheritance was used as material to conduct phenotypic analysis and gene mapping.Phenotypic identification results showed that the leaves of rl-z mutants were inner rolled,forming a tube-like structure throughout the whole growth period.Compared with wild type rice,the plant height,panicle length,number of tillers,and thousand-grain weight of mutant were significantly decreased.Genetic analysis displayed that the mutant phenotype of rl-z was controlled by a pair of recessive genic genes.Gene mapping was performed by using the F2 population produced by crossing rl-z mutant with an indica rice variety Yangdao 6.The rolled-leaf gene was mapped between the InDel markers JY11 and JY14 on chromosome 9,and the physical distance between the two markers was 38.5 kb.There was only one predicted gene(RL9,LOC_Os09g23200)within the region,and this gene encoded a GARP transcription factor.Sequencing results showed that the first exon of RL9 was found to exist a 36 bp deletion(nucleotide position 103 086-103 121)and a single-base mutation(nucleotide position 103 524).Results of gene mapping suggested that the rolled leaf characteristic of rl-z mutant was likely to be caused by the abnormal function of GARP transcription factor owing to the mutation of RL9.
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