Identification and bioinformatics analysis of SPL transcription factor fami-ly members in Lycium ruthenicum
The SQUAMOSA promoter-binding protein-like(SPL)family members are involved in plant growth and devel-opment and stress response.To investigate the salt tolerance molecular mechanism of Lycium ruthenicum SPL family genes under salt stress,the gene family members were identified and analyzed by bioinformatics.The results showed that 20 SPL family mem-bers were screened from transcriptome data of Lycium ruthenicum,and they were divided into eight subfamilies.The results of protein subcellular localization prediction showed that SPL proteins were mainly located in the nucleus,and 20 SPL proteins had SBP conserved domains.The relative molecular weight of SPL family proteins in Lycium ruthenicum was 15 296.08-130 310.8.The isoelectric point was 5.64-9.65,the total number of atoms was 2 106-18 214,the number of amino acids was 133-1 176 aa,and the lipid solubility index was 46.35-87.53.All of them were hydrophilic proteins and unstable proteins.The results of protein structure analysis of SPL family in Lycium ruthenicum showed that random coil and α-helix accounted for a large proportion.The results of expression pattern analysis showed that the SPL family genes of Lycium ruthenicum had different expression trends in different organs under NaCl stress,and LrSPL9 was highly expressed in roots and leaves.The results of this study can provide a reference for further research on salt stress re-sponse of SPL gene family members in Lycium ruthenicum.
万方数据
维普
