黑果枸杞SPL转录因子家族成员鉴定与生物信息学分析
Identification and bioinformatics analysis of SPL transcription factor fami-ly members in Lycium ruthenicum
刘筠 1郭毅 2李效雄 3胡晓桐 1马瑞 1杜雨 1贾西贝 1柳迪 1马彦军1
作者信息
- 1. 甘肃农业大学林学院,甘肃 兰州 730070
- 2. 甘肃林业职业技术学院,甘肃 天水 741020
- 3. 兰州资源环境职业技术大学,甘肃 兰州 730030
- 折叠
摘要
SPL(SQUAMOSA promoter-binding protein-like)家族成员参与植物的生长发育和胁迫响应,为探究黑果枸杞SPL家族基因在盐胁迫下的耐盐分子机制,本研究对其基因家族成员进行了鉴定和生物信息学分析.结果表明,从黑果枸杞转录组数据中共筛选出20个SPL基因家族成员,分为 8 个亚家族,蛋白质亚细胞定位预测结果显示SPL蛋白主要定位于细胞核,20个SPL蛋白均具有SBP保守结构域.黑果枸杞SPL家族蛋白质相对分子量为15 296.08～130 310.8;等电点为5.64～9.65;总原子数为2 106～18 214;氨基酸数量为133～1 176 aa;脂溶指数为46.35～87.53;均为亲水性蛋白质和不稳定蛋白质.黑果枸杞SPL家族蛋白质结构分析结果表明,无规则卷曲和α-螺旋占比较大.表达模式分析结果表明,在NaCl胁迫下不同器官中黑果枸杞SPL家族基因具有不同的表达趋势,LrSPL9 在根和叶中均为高表达.该研究结果可为黑果枸杞SPL基因家族成员盐胁迫响应方面的进一步研究提供参考.
Abstract
The SQUAMOSA promoter-binding protein-like(SPL)family members are involved in plant growth and devel-opment and stress response.To investigate the salt tolerance molecular mechanism of Lycium ruthenicum SPL family genes under salt stress,the gene family members were identified and analyzed by bioinformatics.The results showed that 20 SPL family mem-bers were screened from transcriptome data of Lycium ruthenicum,and they were divided into eight subfamilies.The results of protein subcellular localization prediction showed that SPL proteins were mainly located in the nucleus,and 20 SPL proteins had SBP conserved domains.The relative molecular weight of SPL family proteins in Lycium ruthenicum was 15 296.08-130 310.8.The isoelectric point was 5.64-9.65,the total number of atoms was 2 106-18 214,the number of amino acids was 133-1 176 aa,and the lipid solubility index was 46.35-87.53.All of them were hydrophilic proteins and unstable proteins.The results of protein structure analysis of SPL family in Lycium ruthenicum showed that random coil and α-helix accounted for a large proportion.The results of expression pattern analysis showed that the SPL family genes of Lycium ruthenicum had different expression trends in different organs under NaCl stress,and LrSPL9 was highly expressed in roots and leaves.The results of this study can provide a reference for further research on salt stress re-sponse of SPL gene family members in Lycium ruthenicum.
关键词
黑果枸杞/NaCl胁迫/SPL转录因子/生物信息学分析Key words
Lycium ruthenicum/NaCl stress/SPL transcription factor/bioinformatics analysis引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据