Codon usage bias analysis of cowpea GATL gene family
To clarify the codon usage bias of galacturonic acid transferase-like(GATL)genes in cowpea and influen-cing factors,the 32 members of the cowpea GATL gene family were analyzed in this study.The results showed that most of the codons preferred by the GATL gene family ended with A/T.The effective number of codons(ENc)of the cowpea GATL gene family ranged from 39.29 to 55.77,with an average of 51.06,and the contents of G+C at the third position of syn-ony-mous Sodons(GC3)ranged from 34.10%to 74.60%,indicating that the codon usage bias of the cowpea GATL gene family was weak.The relative synonymous codon usage(RSCU)clustering results were basically consistent with the coding sequence(CDS)clustering results.Results of parity rule 2(PR2)-plot analysis,ENc-plot analysis,and neutral plotting a-nalysis showed that the codon usage bias of cowpea GATL gene family was mainly affected by natural selection.The results of this study provide a theoretical basis for the gene expression and functional verification of GATL in cowpeas.