Changes of phthalic acid ester content,subcellular distribution in apricot fruits at different growth stages and their correlation analysis
To clarify the changes in phthalic acid ester(PAEs)content,subcellular distribution and their correla-tions during different growth stages of apricots,experimental sites were established in six major apricot-producing areas in Xinjiang to collect the samples of apricots at different growth stages.A comprehensive analysis of the contents of PAEs mon-omer compounds in the samples and three subcellular fractions was conducted using methods such as gas chromatography-mass spectrometry and differential centrifugation.Furthermore,the changes of PAEs content and subcellular distribution during different growth stages of apricots and their correlation were investigated.The results indicated that eight PAEs monomer compounds were detected in the sam-ples,with the highest content of the dibutyl phthalate(DBP)monomer.The content of PAEs in apricots during different growth stages and the content of PAEs monomer compounds in subcellular fractions were found to fluctuate.In different subcellular fractions,the distribution pattern of DBP showed soluble fractions>organelles>cell walls,which was inconsistent with the distribution patterns of other monomers.With the increase of sampling time,the changes in the ac-cumulation and proportion of diisobutyl phthalate(DIBP)and dimentyl phthalate(DMP)in the cell walls of apricot fruits at some test points were similar to the changes in the accumulation of PAEs.The results of correlation analysis showed that the contents of butyl benzyl phthalate(BBP),DIBP,DMP and DBP were significantly(P<0.05)or extremely significant-ly(P<0.01)positively correlated with the cell wall fractions,with correlation coefficients of 0.41,0.41,0.55 and 0.55,respectively.Therefore,it can be seen that the high proportion of soluble fractions may be the reason why the content of DBP in apricots is higher than that of other monomers,and the cell wall may be the main subcellular fraction affecting the changes in the content of BBP,DIBP,DMP and DBP in apricots.The aforementioned results can provide a reference for further research on the metabolism and absorption processes of PAEs in apricot fruits.
万方数据
维普
