Effects of active components in different varieties of Euryale ferox on hu-man diabetes based on metabolomics and network pharmacology
This study aims to elucidate the active ingredient differences in Euryale ferox and the alleviation of diabetes a-mong different varieties,known as South Euryale and North Euryale,using metabolomics,network pharmacology,and molecular docking techniques.Liquid chromatography-quadrupole time of flight-mass spectrometry(LC-QTOF-MS),Metaboanalyst(ht-tps://www.metaboanalyst.ca/),and TBtools were used for the detection and analysis of components in E.ferox.Bioactive components in E.ferox were screened through Pub-Chem.Swiss was used to predict the targets of E.ferox com-ponents,and disease targets were searched from multiple da-tabases and combined.Protein-protein interaction(PPI)net-works were constructed to identify major components and core targets.GO analysis and KEGG analysis were conducted on the in-tersected targets.Finally,molecular docking models were used for validation.The results showed that 153 components with signif-icant differences in the content were found between South Euryale and North Euryale.Twelve components with the potential to al-leviate diabetes and its complications,along with 578 potential targets,were identified in South Euryale,while three components and 226 potential targets were identified in North Euryale.GO and KEGG enrichment analysis revealed that the targets in South Euryale were mainly enriched in the pathogenesis of diabetic cardiomyopathy,insulin resistance,and the advanced glycation end products-receptor of advanced glycation endproducts(AGE-RAGE)signaling pathway in diabetic complications,while the targets in North Euryale were primarily enriched in the pathogenesis of cancer,regulation of adipocyte lipolysis,and the adenosine 5'-monophosphate(AMP)-activated protein kinase(AMPK)signaling pathway.Molecular docking results showed that the screened components combined to the depressed sites of target proteins,with negative binding free energy and stable molecular docking conformations.It can be concluded that South Euryale exhibited better effects in alleviating diabetes and its complications com-pared to North Euryale.
万方数据
维普
