Cloning,bioinformatics analysis and expression profiles of TaSRG1 gene in wheat
In order to clarify the biological function of wheat zinc finger protein TaSRG1,TaSRG1 gene was cloned from Chinese spring wheat,and the bioinformatics analysis of its encoded protein was carried out by online software.The ex-pression of TaSRG1 gene in wheat tissues and its response to stresses such as hormones,salt and drought were analyzed by RT-PCR and qRT-PCR.The results showed that the coding sequence(CDS)of TaSRG1 gene in wheat was 1 047 bp in length,and the protein encoded by the gene was a non-secreted hydrophilic stable protein,located in the cytoplasm,con-taining 23 phosphorylation sites.Multiple sequence alignment and phylogenetic tree analysis indicated that the biological function of wheat TaSRG1 gene was most similar to that of emmer wheat.The TaSRG1 promoter region contained 18 cis-act-ing elements,of which five were related to plant hormones.TaSRG1 gene was expressed in roots,stems and leaves of wheat,and the expression level in stems was the highest.The relative expression of TaSRG1 gene in wheat leaves increased first and then decreased at 3-72 h after hormone(ABA,MeJA),salt(NaCl)and drought stress treatments.The results of this study provide a basis for the breeding application of wheat SRG1 gene.
万方数据
维普
