原生质体瞬时转化系统简便高效,已被广泛用于植物基因功能分析.然而,目前甘薯中尚未建立原生质体瞬时转化系统.本研究以徐紫薯 8 号水培根系为试验材料,通过聚乙二醇(PEG)介导,建立了一个操作简便且高效的原生质体分离和瞬时转化系统.结果表明,水培根系制备的原生质体产量高达每 1 g 1.106×107个,质粒转化效率高达 69.46%.并利用该系统,首次成功将甘薯蔗糖转运蛋白(IbSUT4)定位到甘薯细胞膜上.本研究结果为甘薯功能基因研究和分子育种提供了重要技术支撑.
Establishment and application of an efficient protoplast transient transfor-mation system in sweet potato
The protoplast transient transformation system is simple and efficient,and has been widely used in plant gene function analysis.However,the protoplast transformation system has not yet been established in sweet potato.In this study,the hydroponic roots of Xuzishu 8 were used as materials to establish a simple and efficient protoplast isolation and transient transformation system mediated by polyethylene glycol(PEG).The results showed that the yield of protoplasts pre-pared from hydroponic roots was as high as 1.106×107 per gram,and the plasmid transformation efficiency was as high as 69.46%.Using this system,the sweet potato sucrose transporter(IbSUT4)was successfully localized on sweet potato cell membrane for the first time.The results of this study provide important technical support for sweet potato functional gene re-search and molecular breeding.
万方数据
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