Genome-wide identification and expression analysis of ABCB genes in gar-lic(Allium sativum L.)
In order to explore the mechanism of auxin transporter ABCB gene(AsaABCB)in the process of somatic em-bryogenesis of garlic,the whole genome of garlic AsaABCB gene family members was identified by bioinformatics method.The ex-pression pattern of AsaABCB family genes during embryo development was analyzed by real-time fluorescence quantitative PCR(qRT-PCR)using Xusuan 6 as experimental material.The results showed that there were 17 AsaABCB genes in garlic,which were located on seven chromosomes and could be divided into five subgroups.The number of amino acids encoded by AsaABCB gene was 545-1 379,the relative molecular weight was 59 627.17-152 864.21,and the theoretical isoelectric point was 5.72-9.34.AsaABCB4,AsaABCB6 and AsaABCB15 proteins were localized in the cell membrane and cytoplasm,and the remaining AsaAB-CB proteins were only localized in the cell membrane.There were 196 light response elements,48 jasmonic acid response elements,26 abscisic acid response elements and 12 auxin response elements in the promoter region of 17 AsaABCB genes.The relative expression levels of 11 AsaABCB family genes were higher in the embryogenic callus(EC)stage of garlic,and the expression levels of five AsaABCB family genes were higher in the callus(CA)stage,indicating that the family genes may mainly regulate the formation and development of EC and CA during the development of garlic somatic embryos.The results of this study lay a foundation for further exploring the function of AsaABCB genes during garlic somatic embryo development.
万方数据
维普
