Analysis of components and contents of organic acids in fruits of different strawberry germplasm resources
To investigate the accumulation characteristics and diversity of organic acids in strawberry fruits,and to provide a theoretical reference for the scientific evaluation and precise identification of strawberry germplasm resources,this study optimized a method for determining organic acids in strawberry fruits using high-performance liquid chromatography(HPLC)method.Organic acid content was measured in mature fruits from 70 strawberry germplasm samples,covering eight species.The results showed that there were significant differences in total acid content among the eight species,ranked from highest to lowest as follows:Fragaria mandshurica,Fragaria viridis,Fragaria vesca,Fragaria emeiensis,cultivated strawberries(Fragaria×ananassa),Fragaria pentaphylla,Fragaria nilgerrensis,and Fragaria nubicola.The main organic acids in strawberry fruits were citric acid and malic acid.The citric acid content in mature fruits of different germplasm resources varied from 3.039 6 mg/g to 23.787 3 mg/g,and malic acid content ranged from 1.335 9 mg/g to 14.830 3 mg/g.The highest citric acid content was found in Fragaria mandshurica DB3,while the lowest was found in cultivated strawberry Tokun.The highest malic acid content was found in cultivated strawberry Shitouhezi,and the lowest was found in Fragaria mandshurica DB4.The results of correlation analysis showed that total acid content was significantly or significantly positively correlated with both citric acid content and malic acid content,and both total acid content and citric acid content exhibited skewed distributions.The results of cluster analysis not only revealed the correlation of organic acid accumulation among different strawberry species,but also reflected the preferences of breeders from different regions in the screening of organic acid traits.The optimized HPLC method can rapidly separate and identify organic acid components in strawberry fruits.Additionally,the selected germplasm resources with specific organic acid contents can be used as parental materials for breeding new strawberry varieties.
万方数据
维普
