Effects of combined application of biomass ash and bio-bacterial agents on the microbial community in tobacco rhizosphere soil and the control effect on bacterial wilt
In order to explore the effects of biomass ash and different bio-bacterial agents on the microbial community in tobacco rhizosphere soil and the prevention and control of tobacco bacterial wilt,this study first used high-throughput sequencing methods to explore the effects of biomass ash combined with bio-bacterial agents on soil microbial community diversity and micro-bial community structure.The results of α diversity index analysis showed that the application of biomass ash combined with bio-bacterial agents was beneficial to improve the abundance and change degree of microbial flora in tobacco rhizosphere soil.The Shannon index and Simpson index of soil treated with biomass ash combined with Pseudomonas fluorescens agent were the highest,and the Ace and Chao1 values of tobacco rhizosphere soil treated with biomass ash combined with the photosynthetic bacteria a-gent were the highest.Through principal component analysis,it was found that the effects of biomass ash combined with different bio-bacterial agents on rhizosphere soil microorgan-isms were similar.In addition,the relative abundance of Ac-idobacteria or Actinomycetes in soil could be significantly in-creased by the application of biomass ash combined with bio-bacterial agents.The relative abundance of soil actinomycetes was higher under the treatments of biomass ash combined with Pseudomonas fluorescens agent and photosynthetic bacteria agent.The effect of biomass ash combined with bio-bacterial agents on the number of microbial communities was studied by soil microbial separation method.The results revealed that compared with CK,biomass ash combined with bio-bacterial agents could promote the growth and reproduction of soil microorganisms and signif-icantly increase the number of microbial communities.The biomass ash combined with the photosynthetic bacteria agent had the most significant effect on the growth and reproduction of microorganisms.The disease index of tobacco bacterial wilt after biomass ash combined with bio-bacterial agents treatment was significantly lower than that of CK.In particular,the disease index of tobac-co bacterial wilt treated with biomass ash combined with the photosynthetic bacteria agent was the lowest and the control effect was the best.
万方数据
维普
