Composition and diversity of microbial community in rhizosphere soil of Edgeworthia chrysantha Lindl.with root rot disease
Root rot is one of the important diseases affecting the sustainable development of Edgeworthia chrysantha Lindl.industry.To explore the pathogenesis of root rot of Edgeworthia chrysantha Lindl.,screen potential pathogens,and develop effective control agents,this study took the Edgeworthia chrysantha Lindl.forest with high incidence of root rot in Qianshan City,Anhui province as the research object,and used high-throughput sequencing technology(IlluminaMiSeq)to analyze and compare the rhizosphere soil physical and chemical properties,bacterial and fungal community structure and diversity of healthy plants and diseased plants.The results showed that compared with healthy plants,the bacterial richness index(Chao1 index)and fungal richness index(Chao1 index)in rhizosphere soil of diseased plants were significantly re-duced by 13.74%and 45.83%,respectively,and the bacterial diversity(Shannon index)did not change significantly,but the fungal diversity(Shannon index)was significantly increased.Compared with the rhizosphere soil of healthy plants,the relative abundance of Byssovorax in the rhizosphere soil of diseased plants increased signifi-cantly(P<0.05),and the relative abundance of Botryo-sphaeria and Sebacinales_unclassified increased significantly(P<0.01).The organic carbon content in rhizosphere soil of diseased plants was significantly higher than that of healthy plants.In summary,after the infection of root rot,the richness of bacteria and fungi in rhizosphere soil decreased,and the diversity of fungal community increased.It was preliminarily determined that Byssovorax,Botryosphaeria and Sebacinales_unclassified may be the pathogens causing root rot.The results of this study provide a candidate flora for the subsequent isolation and culture of pathogenic bacteria.
万方数据
维普
