Effects of mixed sowing of different types of green manure on soil fungal community structure and physicochemical properties
In order to clarify the effects of mixed sowing of different types of green manure on soil fungal community structure and its main driving factors in Qinghai area,a comparative experiment of mixed sowing of common vetch and hairy vetch(HV),mixed sowing of common vetch and highland barley(HB),mixed sowing of common vetch and rape(RS)and single sowing control of common vetch(CK)was set up in this study.The effects of mixed sowing of different types of green manure on soil basic physical and chemical proper-ties,organic carbon components,fungal community diversi-ty and composition were analyzed by metagenomic sequen-cing technology.The results showed that compared with CK,the soil organic carbon content increased by 10.84%under HV treatment,and the soil microbial biomass carbon content increased by 19.97%under RS treatment.The richness(Chaol index)and diversity(Simpson index)of soil fungal communities in RS treatment were 36.42%and 11.59%higher than those in CK,respectively.The mixed sowing of different types of green manure could also increase the richness of soil fungal community composition.At the phylum level,the relative abundance of Mucoromycota and Basidiomycota in HB treat-ment increased by 7.24%and 113.09%,respectively.The relative abundance of Chytridiomycota in RS treatment increased by 583.79%.Soil total carbon content and microbial biomass carbon content were significantly correlated with soil fungal commu-nity structure,which were the main driving factors affecting soil fungal community structure and diversity.The results of this study showed that green manure mixed sowing could better optimize soil environment and fungal community structure than green manure monoculture.
green manuremixed sowingsoil fungal communitysoil physicochemical properties