Promotion of alliin accumulation by endogenous Bacillus velezensis in garlic
A mutually beneficial symbiotic relationship happens between endophytic bacteria and plants,which can promote the accumulation of active substances in the host plant.To investigate whether endophytic bacteria of garlic promote the accumulation of alliin,an active substance in garlic,Bacillus velezensis KLBMP108 and Bacillus cereus KLBMP81 were used as the test strains to determine their effects on the alliin content in leaves of three different varieties of garlic in differ-ent regions.The results showed that compared with the control,on the 6th day after treatment,the strain KLBMP108 could increase the alliin content of the improved garlic to 1.68 mg/mL,and the expression of alliinase was significantly increased.However,there was no significant effect on the alliin content of non-host varieties Henan purple garlic and Yunnan moss garlic.The alliin content of three kinds of garlic treated by strain KLBMP81 isolated from Taxus chinensis was low.In con-clusion,strain KLBMP108 can increase the alliin content in its host Gailiang garlic leaves,and is a valuable microbial resource for garlic variety improvement.