Regulation of cilia in G1 phase on the susceptibility of host cells to PEDV
This study aimed to research on the regulation of host cell cycle on porcine epidemic diarrhea virus(PEDV)pro-liferation.Swine testis(ST)and intestinal porcine epithelial cell line-J2(IPEC-J2)cells were infected using PEDV with multiplicity of infection(MOI)=1,and the cell cycle was identified by fluorescence activated cell sorting(FACS)and Western blot(WB).Cili-ary assembly was observed by immunofluorescence.Medium tissue culture infective dose(TCID50)was measured to identify virus pro-liferation ability.PEDV infected ST cells and IPEC-J2 cells,and the host cells were arrested at the synthesis phase(S phase)and the pre-synthesis phase(G1 phase)after infection of 7 h and 31 h,respectively.FACS and WB tests confirmed that the use of doub-le-thymidine release in coordination with nocodazole could block the cell cycle in the G1-S transition phase,S phase and cell division phase(M phase),respectively.PEDV(MOI=1)infected synchronized ST cells and IPEC-J2 cells,and the TCID50 of cells synchronized in the G1-S transition phase was the highest after PEDV infection.Cells with ciliary disassembly were less sensitive to PEDV.PEDV infection arrested cell cycle to G1-S transition.Cells in G1 phase were most conducive to PEDV proliferation due to cilia.
万方数据
维普
