This study aimed to research on the regulation of host cell cycle on porcine epidemic diarrhea virus(PEDV)pro-liferation.Swine testis(ST)and intestinal porcine epithelial cell line-J2(IPEC-J2)cells were infected using PEDV with multiplicity of infection(MOI)=1,and the cell cycle was identified by fluorescence activated cell sorting(FACS)and Western blot(WB).Cili-ary assembly was observed by immunofluorescence.Medium tissue culture infective dose(TCID50)was measured to identify virus pro-liferation ability.PEDV infected ST cells and IPEC-J2 cells,and the host cells were arrested at the synthesis phase(S phase)and the pre-synthesis phase(G1 phase)after infection of 7 h and 31 h,respectively.FACS and WB tests confirmed that the use of doub-le-thymidine release in coordination with nocodazole could block the cell cycle in the G1-S transition phase,S phase and cell division phase(M phase),respectively.PEDV(MOI=1)infected synchronized ST cells and IPEC-J2 cells,and the TCID50 of cells synchronized in the G1-S transition phase was the highest after PEDV infection.Cells with ciliary disassembly were less sensitive to PEDV.PEDV infection arrested cell cycle to G1-S transition.Cells in G1 phase were most conducive to PEDV proliferation due to cilia.
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