Identification and expression analysis of SOD family genes in four kinds of grapes
Superoxide dismutase(SOD)plays an important role in the balance between oxidation and antioxidation in plants.In this study,the whole genome identification and expression analysis of SOD family genes in four kinds of grapes were carried out.The identification results showed that there were 45 SOD genes in the four kinds of grapes,including 12 genes in Pinot Noir,eight genes in Vitis amurensis,15 genes in Vitis riparia and 10 genes in Vitis rotundifolia.The SOD genes were randomly distributed on chromosomes.There were many cis-acting elements in the promoter of grape SOD gene,such as hormones,growth and development,stress response and so on.The results of intraspecific collinearity analysis showed that there was only one pair of collinearity in the SOD genes of Pinot Noir,Vitis amurensis and Vitis riparia,while there were six pairs of collinearity in the SOD genes of Vitis rotundifolia.The results of qRT-PCR showed that the VaSOD gene responded significantly to different abiotic stresses such as sodium chloride(NaCl),salicylic acid(SA),abscisic acid(ABA)and low temperature(4℃).The base sequence of SOD genes in four kinds of grapes was relatively conservative and the genetic relationship was rel-atively close.The results of this study can lay a foundation for further analysis of the mechanism of grape SOD family genes in their growth and development and abiotic stress response.
万方数据
维普
