Leaf callus and suspension cell tissue culture of Lavandula angustata Mill.and their metabolite differences
In order to clarify the ability of Lavandula angustifolia Mill.leaves to produce bioactive metabolites,the sterile seedlings of L.angustifolia were used as explants to establish the culture system of callus and suspension cells of sterile seedling leaves.The metabolites of callus and suspension cells were characterized by ultra-high performance liquid chromatog-raphy-quadrupole-time-of-flight tandem mass spectrometry(UHPLC-Q-TOF-MS),and the metabolic pathways of differential metabolites were analyzed.The scavenging ability of metabolites to ABTS(2,2′-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid)free radicals was compared.The results showed that the aseptic seedlings could be obtained after 30 days of culture,and the growth of callus and suspension cells could be increased by more than seven times after eight days of culture.A total of 1 403 metabolites were detected in callus and suspension cells,including rosmarinic acid,oleanolic acid,caffeic acid methyl ester and other characteristic ac-tive substances of lavender and unreported ginsenoside F3,asiatic acid,hydroxyasiatic acid,echinocystic acid and other ac-tive substances.A total of 218 significantly different metabolites were screened from 1 403 metabolites,including 49 bioactive substances.Callus and suspension cells showed different ability to synthesize metabolites.Callus preferred the synthesis of phenolic acids,while suspension cells preferred the synthesis of terpenoids.The differential metabolic pathways were mainly enriched in Metabolic pathways and Nucleotide metabolism pathways.The ethanol extracts of callus and suspension cells had high ABTS free radical scavenging ability,and the half scavenging mass concentration(IC50)was 0.50 mg/mL and 0.49 mg/mL,respectively.Therefore,using the leaves of Lavandula angustata aseptic seedlings as explants,using different tissue culture methods,a variety of secondary metabolites can be obtained.The results of this study can provide a basis for callus and suspension cell tissue culture and metabolite extraction of Lavender angustifolia leaves.
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