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杉木不同器官中内生细菌的多样性分析

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为探明杉木不同器官中内生细菌的群落组成和特点,挖掘杉木丰富的微生物资源,并为筛选杉木中特色微生物资源提供理论依据,本研究采用Illumina Miseq平台的高通量测序技术,对多年生杉木根、茎、叶中的内生细菌 16S rDNA V3~V4 高变区序列进行检测,分析杉木不同器官中内生细菌的多样性及其群落组成差异.扩增子序列变体(ASV)分析结果表明,杉木根、茎、叶中的ASV数量分别为7 108个、2 017个和1 911个.对杉木根、茎、叶中内生细菌进行分析,发现变形菌门(Proteobacteria)、放线菌门(Actinobacteria)和酸杆菌门(Acidobacteria)为主要优势菌门,相对丰度分别为 67.930%、13.800%和 6.250%;α-变形菌纲(Alphaproteobacteria)、γ-变形菌纲(Gamma-proteobacteria)和放线菌纲(Actinobacteria)为主要优势菌纲,相对丰度分别为 41.180%、19.710%和 11.180%;1174-901-12、P3OB-42 和甲基杆菌属(Methylobacterium)为主要优势菌属,相对丰度分别为 14.430%、5.890%和 5.300%.物种组成热图与主坐标分析(PCoA)结果表明,杉木根中的内生细菌菌群组成与茎、叶中的内生细菌菌群组成存在明显差异,茎与叶中的内生细菌菌群组成相似.功能预测结果表明,多年生杉木根、茎、叶中内生细菌的代谢途径主要为生物合成.多年生杉木根、茎、叶中存在丰富的内生细菌,根中的内生细菌多样性、丰富度高于茎和叶.本研究揭示了杉木内生细菌的群落组成,可以为今后筛选、利用杉木内生细菌资源提供理论依据.
Diversity analysis of endophytic bacteria in different organs of Cunning-hamia lanceolata(Lamb.)Hook.
To explore the community composition and characteristics of endophytic bacteria in different organs of Cun-ninghamia lanceolata(Lamb.)Hook.,explore the rich microbial resources of C.lanceolata,and provide a theoretical basis for screening the characteristic microbial resources in C.lanceolata,the sequences of endophytic bacterial 16S rDNA V3-V4 high-variable regions in roots,stems and leaves of the perennial C.lanceolata were detected using high-throughput sequencing technol-ogy based on the Illumina Miseq platform.Moreover,the diversity and community structure of endophytic bacteria in different or-gans of C.lanceolata were analyzed.The results of amplicon sequence variant(ASV)analysis showed that the number of ASV in C.lanceolata roots,stems and leaves was 7 108,2 017 and 1 911,respectively.Proteobacteria,Actinobacteria and Acidobacteria were the dominant bacteria in different or-gans of C.lanceolata,the relative abundances were 67.930%,13.800%and 6.250%.Alphaproteobacteria,Gammaproteobacteria and Actinobacteria were the main dominant classes,with rela-tive abundances of 41.180%,19.710%and 11.180%,respectively.1174-901-12,P3OB-42 and Methylobacterium were the main dominant genera,with relative abundances of 14.430%,5.890%and 5.300%,respectively.The results of species composition heat map and principal coordinate analysis(PCoA)indicated that the composition of endophytic bacteria in the roots of C.lan-ceolata was significantly different from that in the stems and leaves,and the composition of endophytic bacteria in the stems and leaves was similar.The functional prediction results showed that the metabolic pathway of endophytic bacteria in roots,stems and leaves of perennial C.lanceolata was mainly biosynthesis.Rich endophytic bacteria were present in roots,stems and leaves of pe-rennial C.lanceolata,and the diversity and abundance of endophytic bacteria in roots were higher than those in stems and leaves.This study reveals the community composition of endophytic bacteria in C.lanceolata,which can provide a theoretical basis for the screening and utilization of endophytic bacteria resources in C.lanceolata in the future.

Cunninghamia lanceolata(Lamb.)Hook.endophytic bacteriahigh-throughput sequencingdiver-sityrelative abundance

孟磊、林晗、丁新兰、谢安强、简灵静、王英姿、陈灿

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福建农林大学林学院,福建 福州 350002

福建省高校森林生态系统经营与过程重点实验室,福建 福州 350002

福建省上杭白砂国有林场,福建 龙岩 364200

福建农林大学菌草与生态学院,福建 福州 350002

福州市自然保护地规划发展中心,福建 福州 350002

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杉木 内生细菌 高通量测序 多样性 相对丰度

2024

江苏农业学报
江苏省农业科学院

江苏农业学报

CSTPCD北大核心
影响因子:1.093
ISSN:1000-4440
年,卷(期):2024.40(12)