Diversity analysis of endophytic bacteria in different organs of Cunning-hamia lanceolata(Lamb.)Hook.
To explore the community composition and characteristics of endophytic bacteria in different organs of Cun-ninghamia lanceolata(Lamb.)Hook.,explore the rich microbial resources of C.lanceolata,and provide a theoretical basis for screening the characteristic microbial resources in C.lanceolata,the sequences of endophytic bacterial 16S rDNA V3-V4 high-variable regions in roots,stems and leaves of the perennial C.lanceolata were detected using high-throughput sequencing technol-ogy based on the Illumina Miseq platform.Moreover,the diversity and community structure of endophytic bacteria in different or-gans of C.lanceolata were analyzed.The results of amplicon sequence variant(ASV)analysis showed that the number of ASV in C.lanceolata roots,stems and leaves was 7 108,2 017 and 1 911,respectively.Proteobacteria,Actinobacteria and Acidobacteria were the dominant bacteria in different or-gans of C.lanceolata,the relative abundances were 67.930%,13.800%and 6.250%.Alphaproteobacteria,Gammaproteobacteria and Actinobacteria were the main dominant classes,with rela-tive abundances of 41.180%,19.710%and 11.180%,respectively.1174-901-12,P3OB-42 and Methylobacterium were the main dominant genera,with relative abundances of 14.430%,5.890%and 5.300%,respectively.The results of species composition heat map and principal coordinate analysis(PCoA)indicated that the composition of endophytic bacteria in the roots of C.lan-ceolata was significantly different from that in the stems and leaves,and the composition of endophytic bacteria in the stems and leaves was similar.The functional prediction results showed that the metabolic pathway of endophytic bacteria in roots,stems and leaves of perennial C.lanceolata was mainly biosynthesis.Rich endophytic bacteria were present in roots,stems and leaves of pe-rennial C.lanceolata,and the diversity and abundance of endophytic bacteria in roots were higher than those in stems and leaves.This study reveals the community composition of endophytic bacteria in C.lanceolata,which can provide a theoretical basis for the screening and utilization of endophytic bacteria resources in C.lanceolata in the future.