Genetic identification and phylogenetic analysis of Ginkgo biloba male plants using ISSR molecular marker
ISSR molecular markers were used for phylogenetic analysis among 42 male plants of Ginkgo biloba.12 polymorphic primers were screened from 100 primers,and a total number of 94 bands were amplified using these primers,among which 56 bands were polymorphic and average bands for each primer pair were 7.83,and the polymorphism ratio was 59.57 ％.The above results suggested that the genetic diversity was higher among Ginkgo males.Cluster analysis was performed by the method of UPGMA,and 42 male ginkgo plants were divided into 3 groups at the genetic similarity coefficient level of 0.51.A three-dimensional map representing relationships among male plants was also established by principal coordinate analysis with the EIGEN method.The two kinds of classification methods above exhibited similar results that the phylogenetic relationship was not completely correlated with regional distribution.Genetic relationship of partial male plants between Shandong and Jiangsu were relatively closer.
国家科技期刊平台
NSTL
万方数据
维普
