A 16S rRNA-based study of the effect of copper contamination on antibiotic resistance genes and horizontal gene transfer in soil bacterial communities
In this study,soil samples were collected from three planting ponds treated with 0,200 and 400 mg Cu2+,respectively,and all samples were sequenced by 16S rRNA high-throughput sequencing.Sequencing results showed that Cu contamination changed the bacterial community structure in soil,and the abundance of antibiotic resistance genes(ARGs)and horizontal gene transfer(HGT)related genes increased with the increase of Cu2+concentration.The resul-ting antibiotic resistance genes were mostly multidrug resistance genes.This study showed that under the stress of Cu contamination,the bacterial community structure in soil was changed,and the HGT between bacteria was promoted,while the ARGs were enriched.
copper contaminationbacterial community structureantibiotic resistance geneshorizontal gene transfer