Preparation,characterization and preliminary in vitro antitumor evaluation of artemisinin-acetone layer co-assembled nanomedicines
To solve the problem of poor water solubility of artemisinin and its acetone layer(industrial extraction waste of artemisinin with polymethoxyflavonoids as the main component)which leads to the limitation of its antitumor applica-tion,the supramolecular co-assembled nanomedicine of artemisinin and acetone layer with homogeneous quality and stable structure was constructed by using the method of emulsification-solution volatilisation.Laser particle size apparatus,fou-rier transform infrared spectrometer and scanning electron microscope were applied to characterize them,and MTT and scratch assay were used to determine the effects of nanomedicines on the proliferation and migration ability of mouse-de-rived CT26 colon cancer cells.The results showed that the average particle size of the prepared nanomedicine was 106.93 nm,the drug loading capacity was 7.78％,and the encapsulation rate was 72％;the IC50 of the co-assembled drug(artemisinin∶acetone layer=1∶2)was artemisinin∶acetone layer=17.13 mg·L-1∶34.27 mg·L-1,which was significantly better than that of the single-use group of the drugs,and the scratching experiment demonstrated that the co-assembled drug had a significant inhibition effect on the migration of the tumor cells.In conclusion,the supramolecular co-assembled nanomedicine of artemisinin-acetone layer prepared in this study has strong anti-tumor activity,which lays an important foundation for the development of anti-tumor drugs and the recycling of traditional Chinese medicine extract waste.
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