Exercise for weight loss improves oocyte viability and early embryonic development in obese mice
In order to investigate the effect of obesity on the fertility of female animals and to explore the feasibility of improving the effect of obesity on reproduction through the exercise of swimming for weight loss,three-week-old C57BL/6 mice were divided into three groups:1)high-fat diet(HFD),2)sprot exercise to lose weight(SE),and 3)normal diet(ND).The effects of obesity on mouse fertility were assessed by counting the ovarian coefficient,the number of mice ovulating after supernumerary ovulation,the maturation rate of oocytes in vivo and in vitro,the rate of early embryonic develop-ment after in vitro fertilization,the structural integrity of spindle in oocytes,and the homogeneity of mitochondrial distri-bution.The results showed that the HFD group was significantly lower than the ND group and the SE group in these in-dexes of ovarian coefficient including number of supernumerary ovulations,in vitro maturation rate of eggs,in vitro mat-uration rate,and number of follicles in the ovaries(P＜0.05),and there was no significant difference between the ND and SE groups(P＞0.05).The spindle immunofluorescence results showed that the percentage of normal spindle in MII stage oocytes of mice in the HFD group[(22.94±4.73)％]was significantly lower than that in the ND group[(85.63± 6.63)％]and the SE group[(81.05±5.5)％].Mitochondrial immunofluorescence results showed that the percentage of homogeneous mitochondrial distribution in mice in the HFD group[(25.73±13.42)％]was significantly lower than that in the ND group[(50.77±3.08)％]and the SE group[(52.05±7.15)％].The in vitro fertilization rate and early em-bryonic development rate of eggs in the HFD group were significantly lower than those in the ND and SE groups.These results indicate that obesity negatively affects oocyte quality and early embryonic development in mice,and that weight loss through swimming exercise can ameliorate the negative effects induced by obesity,and the research work provides an experimental basis for subsequent in-depth studies on the effects of obesity on the fertility of female animals.
obesityexercise for weight lossoocyte qualityembryonic developmenthigh-fat diet
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