Species identification and quality evaluation of tea based on HPLC fingerprint and stoichiometry
To establish the high performance liquid chromatography(HPLC)fingerprint of tea and a stoichiometric method for tea species identification and quality evaluation.The contents of 9 components including gallic acid,theobro-mine,gallocatechin,epigallocatechin,caffeine,epicatechin,epigallocatechin gallate,gallocatechin gallate and epicatechin gallate in 16 batches of tea samples were determined by HPLC.The similarity analysis,cluster analysis,principal compo-nent analysis and discriminant analysis were used to compare and evaluate the quality of tea.The results showed that 20 common fingerprint peaks were calibrated by HPLC fingerprint of tea,9 components were identified.The contents of 9 components in different varieties of tea were different.Stoichiometric analysis showed that 16 batches of tea samples could be divided into 3 categories.In conclusion,this method can provide reference for species identification and quality evaluation of tea.