首页|3种商品茶多酚的表征、抗氧化性及其抑菌效果比较研究

3种商品茶多酚的表征、抗氧化性及其抑菌效果比较研究

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  • 国家科技期刊平台
  • NETL
  • NSTL
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以3种商品茶多酚(CTP)为研究对象,对其儿茶素类化合物组分及其结构进行表征,并探究其抗氧化性和抑菌效果.液相质谱测定结果表明,3种CTP均检出7种儿茶素类化合物,其中表没食子儿茶素没食子酸酯(EGCG)含量最高,CTP1-CTP3含量分别为40.12％、36.27％和59.13％.SEM图谱分析发现,3种CTP均呈现出大小不一和棱角分明的块状结构.红外光谱分析显示,3种CTP分别在1 342～1 312和1 141 cm-1处出现酚羟基的特征峰,且以CTP3峰值强度最高,依次高于CTP1和CTP2.CTP3对DPPH、·OH、ABTS自由基的半抑制浓度最低,分别为0.087 6、0.060 5、0.152 5 mg·mL-1.CTP3对金黄色葡萄球菌和沙门菌的抑菌效果最佳,其抑菌圈直径分别为23.24和11.43 mm.这一研究为CTP作为抗氧化剂和抑菌剂在食品加工中的开发和应用提供了参考.
A comparative study on the characterizations,antioxidant properties and antibacterial effects of three commercial tea polyphenols
Three commercial tea polyphenols(CTP)were used as research objects to characterize their catechin-based compound components and characterizations,and their antioxidant activities and antibacterial effects were further ex-plored.Liquid phase mass spectrometry's result indicated that,7 kinds of catechin-based compounds were detected in all three CTP,with the highest relative content of epigallocatechin gallate(EGCG)component being 40.12％,36.27％and 59.13％for CTP1-CTP3,respectively.SEM test found that,all three CTP exhibited block structures with different si-zes and sharp edges.Infrareds pectrum analysis showed that,the characteristic peaks of phenolic hydroxyl group occurred at the respective 1 342-1 312 and 1 141 cm-1 for all three CTP,and their peak intensity sequence followed CTP3＞CTP1＞CTP2.Half inhibition concentration of CTP3 to DPPH,·OH and ABTS free radical were lowest with 0.087 6,0.060 5 and 0.152 5 mg·mL-1,respectively.CTP3 possessed the best antibacterial effects on the Staphylococcus aureus and Salmo-nella with 23.24 and 11.43 mm of inhibitory zone diameter,respectively.This research results could provide a theoretical reference for CTP as antioxidants and antibacterial agents in the development and application of food processing.

tea polyphenolsliquid phase mass spectrometryinfrared spectrumfree radical scavenginginhibition zone

刘雅楠、张迅、单洪雁、赵文鹏、李松南、王君

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扬州大学旅游烹饪学院,江苏扬州 225127

扬州大学农业科技发展研究院/教育部农业与农产品安全国际合作联合实验室,江苏扬州 225009

茶多酚 液相质谱 红外光谱 自由基清除 抑菌圈

国家自然科学基金江苏省自然科学基金中国博士后科学基金面上项目扬州市重点研发计划(社会发展)项目扬州市绿扬金凤计划扬州大学大学生科技创新基金

82000791BK202205852022M712692YZ2022076YZLYJFJH2021YXBS172X20220894

2024

10.16872/j.cnki.1671-4652.2024.02.010

扬州大学学报(农业与生命科学版)
扬州大学

扬州大学学报(农业与生命科学版)

CSTPCD北大核心
影响因子：0.924
ISSN：1671-4652
年,卷(期)：2024.45(2)
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