3种商品茶多酚的表征、抗氧化性及其抑菌效果比较研究
A comparative study on the characterizations,antioxidant properties and antibacterial effects of three commercial tea polyphenols
刘雅楠 1张迅 1单洪雁 1赵文鹏 1李松南 2王君1
作者信息
- 1. 扬州大学旅游烹饪学院,江苏扬州 225127
- 2. 扬州大学农业科技发展研究院/教育部农业与农产品安全国际合作联合实验室,江苏扬州 225009
- 折叠
摘要
以3种商品茶多酚(CTP)为研究对象,对其儿茶素类化合物组分及其结构进行表征,并探究其抗氧化性和抑菌效果.液相质谱测定结果表明,3种CTP均检出7种儿茶素类化合物,其中表没食子儿茶素没食子酸酯(EGCG)含量最高,CTP1-CTP3含量分别为40.12％、36.27％和59.13％.SEM图谱分析发现,3种CTP均呈现出大小不一和棱角分明的块状结构.红外光谱分析显示,3种CTP分别在1 342～1 312和1 141 cm-1处出现酚羟基的特征峰,且以CTP3峰值强度最高,依次高于CTP1和CTP2.CTP3对DPPH、·OH、ABTS自由基的半抑制浓度最低,分别为0.087 6、0.060 5、0.152 5 mg·mL-1.CTP3对金黄色葡萄球菌和沙门菌的抑菌效果最佳,其抑菌圈直径分别为23.24和11.43 mm.这一研究为CTP作为抗氧化剂和抑菌剂在食品加工中的开发和应用提供了参考.
Abstract
Three commercial tea polyphenols(CTP)were used as research objects to characterize their catechin-based compound components and characterizations,and their antioxidant activities and antibacterial effects were further ex-plored.Liquid phase mass spectrometry's result indicated that,7 kinds of catechin-based compounds were detected in all three CTP,with the highest relative content of epigallocatechin gallate(EGCG)component being 40.12％,36.27％and 59.13％for CTP1-CTP3,respectively.SEM test found that,all three CTP exhibited block structures with different si-zes and sharp edges.Infrareds pectrum analysis showed that,the characteristic peaks of phenolic hydroxyl group occurred at the respective 1 342-1 312 and 1 141 cm-1 for all three CTP,and their peak intensity sequence followed CTP3＞CTP1＞CTP2.Half inhibition concentration of CTP3 to DPPH,·OH and ABTS free radical were lowest with 0.087 6,0.060 5 and 0.152 5 mg·mL-1,respectively.CTP3 possessed the best antibacterial effects on the Staphylococcus aureus and Salmo-nella with 23.24 and 11.43 mm of inhibitory zone diameter,respectively.This research results could provide a theoretical reference for CTP as antioxidants and antibacterial agents in the development and application of food processing.
关键词
茶多酚/液相质谱/红外光谱/自由基清除/抑菌圈Key words
tea polyphenols/liquid phase mass spectrometry/infrared spectrum/free radical scavenging/inhibition zone引用本文复制引用
基金项目
国家自然科学基金(82000791)
江苏省自然科学基金(BK20220585)
中国博士后科学基金面上项目(2022M712692)
扬州市重点研发计划(社会发展)项目(YZ2022076)
扬州市绿扬金凤计划(YZLYJFJH2021YXBS172)
扬州大学大学生科技创新基金(X20220894)
出版年
2024
国家科技期刊平台
NSTL
万方数据