摘要
水稻是最重要的粮食作物之一,干旱、高温、盐等不利因素均会严重影响水稻产量.脱落酸(ABA)作为一种胁迫响应激素,参与调控多种非生物胁迫应答.以ABA信号组分A亚族2C类蛋白磷酸酶基因OsPP2C49为研究对象,探究其调控水稻干旱和高温胁迫的响应机制.结果表明:敲除OsPP2C49基因显著提高水稻在干旱和高温条件下的存活率,而过量表达会导致存活率显著降低.OsPP2C49的表达可被不同逆境胁迫诱导.OsbZIP10、OsbZIP40和OsbZ-IP42可结合OsPP2C49的启动子,激活OsPP2C49的转录.OsbZIP10、OsbZIP40和OsbZIP42的表达亦可被干旱和高温显著诱导,推测OsPP2C49在胁迫条件下的诱导表达是由不同bZIP转录因子介导的.这一研究可为培育耐旱和耐高温的水稻品种提供新的遗传信息.
Abstract
Rice is a crucial food crop,and its yield is significantly affected by adverse environmental conditions,inclu-ding drought,high-temperature,and salinity.ABA,a stress-responsive hormone,plays a crucial role in regulating sever-al adversity stress responses.In this study,we focused on the ABA signaling component,OsPP2C49,a member of the clade A protein phosphatase 2C family and found that OsPP2C49 negatively regulated drought and high temperature stress responses.Knockout of OsPP2C49 significantly improved the survival rate of rice under drought and high-temperature conditions,whereas overexpression of OsPP2C49 resulted in a significant decrease in survival rate.Notably,the expres-sion of OsPP2C49 was induced by various adversity stresses.Furthermore,OsbZIP10,OsbZIP40,and OsbZIP42 tran-scription factors binding to the OsPP2C49 promoter,thereby activating its transcription.Under drought and high-tem-perature conditions,the expression of these bZIP transcription factors was also significantly induced,suggesting that the stress-induced expression of OsPP2C49 was likely to be mediated by different bZIP transcription factors.The results will be useful for breeding rice varieties that are resistant to drought and high-temperature.
基金项目
国家自然科学基金青年基金项目(32100259)
江苏省大学生创新创业训练计划项目(202211117048Z)
国家科技期刊平台
NSTL
万方数据