Mechanism of the rice type 2C protein phosphatase gene OsPP2C49 in regulating drought and high-temperature stress responses
Rice is a crucial food crop,and its yield is significantly affected by adverse environmental conditions,inclu-ding drought,high-temperature,and salinity.ABA,a stress-responsive hormone,plays a crucial role in regulating sever-al adversity stress responses.In this study,we focused on the ABA signaling component,OsPP2C49,a member of the clade A protein phosphatase 2C family and found that OsPP2C49 negatively regulated drought and high temperature stress responses.Knockout of OsPP2C49 significantly improved the survival rate of rice under drought and high-temperature conditions,whereas overexpression of OsPP2C49 resulted in a significant decrease in survival rate.Notably,the expres-sion of OsPP2C49 was induced by various adversity stresses.Furthermore,OsbZIP10,OsbZIP40,and OsbZIP42 tran-scription factors binding to the OsPP2C49 promoter,thereby activating its transcription.Under drought and high-tem-perature conditions,the expression of these bZIP transcription factors was also significantly induced,suggesting that the stress-induced expression of OsPP2C49 was likely to be mediated by different bZIP transcription factors.The results will be useful for breeding rice varieties that are resistant to drought and high-temperature.
riceabscisic acidtype 2C protein phosphataseOsPP2C49drought tolerancehigh-temperature tolerance
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