摘要
为研究荞麦(Fagopyrum esculentum Moench)自然抗性相关巨噬细胞蛋白(NRAMP)家族成员结构与功能,借助生物信息学手段,从荞麦全基因组数据库中筛选并鉴定荞麦NRAMP家族基因序列,进而对该家族成员的蛋白质理化性质、基因结构、保守结构域、保守基序、亚细胞定位、磷酸化位点、系统进化关系、二级结构和三级结构进行分析.结果表明:在荞麦全基因组中共鉴定出10个NRAMP基因,命名为FeNRAMP1-FeNRAMP10,多数荞麦NRAMP家族成员蛋白质的氨基酸数介于361～605个,跨膜结构数4～12不等,且亚细胞定位除FeNRAMP10定位于液泡膜上外,其他均定位于细胞膜上.进化树分析结果表明,在进化上越接近于FeNRAMP,其基因结构和保守基序也更为相似.除FeNRAMP9蛋白不含有苏氨酸(Thr)磷酸化位点外,其余9个FeNRAMP蛋白的氨基酸序列均含有潜在的丝氨酸(Ser)、苏氨酸(Thr)和酪氨酸(Tyr)磷酸化位点,大部分荞麦NRAMP蛋白的二级结构形式主要是α-螺旋.启动子元件分析表明,多数荞麦NRAMP基因启动子含有参与基因转录、光响应、激素响应等元件.
Abstract
In order to study the structure and function of NRAMP(natural resistance associated macrophage protein)family members in buckwheat,the NRAMP family genes of buckwheat were screened and identified by bioinformatics methods from the Buckwheat Genome DataBase(BGDB),and then the protein physicochemical properties,gene struc-tures,evolutionary relationship,conserved domains,conserved motifs,subcellular localizations,phosphorylation sites,and the secondary and tertiary structures were analyzed.The results showed that a total of 10 NRAMP genes were identi-fied from the buckwheat genome.The number of amino acids in most members of the buckwheat NRAMP family proteins ranged from 361 to 605,and the number of transmembrane structures ranged from 4 to 12,and the subcellular localization was located on the cell membrane,except for FeNRAMPI0,which was located on the vacuolar membrane.The closer the evolutionary relationship between NRAMP family,the more similar the gene structures and conserved motifs were.The number of transmembrane structures of each member varied.Except for the FeNRAMP9 protein,which did not contain a Thr phosphorylation site,all FeNRAMP proteins contained Ser,Thr and Tyr phosphorylation sites.The main secondary structure of most NRAMP proteins of buckwheat were α-helix.The analysis of promoter elements showed that most of the NRAMP gene promoters of buckwheat contained elements involved in gene transcription,light response,hormone re-sponse and so on.
基金项目
河南省高等学校重点科研项目(23B320011)
河南省社科联课题项目(SKL-2023-1057)
新乡医学院三全学院2022年度校级骨干教师培育项目(SQ2022GGJS01)
新乡医学院三全学院2022年度校级骨干教师培育项目(SQ2022GGJS02)
新乡医学院三全学院2022年度校级优秀青年教师培育项目(SQ2022YQJH02)
国家科技期刊平台
NSTL
万方数据