A model of airway inflammation in asthma mice induced by shrimp tropomyosin plus lipopolysaccharide
Aim to definite the type of asthma model induced by lipopolysaccharide(LPS)combined with allergens.We established a mouse model of asthmatic airway inflammation induced by shrimp tropomyosin(ST)plus LPS and compared this model with models induced by ovalbumin(OVA)plus complete Freund's adjuvant(CFA)for neutrophilic asthma or ST combined with aluminum adjuvant for eosinophilic asthma.Compared to the control group,ST plus LPS induced the increased airway inflammation,airway epithelial mucus secretion,and total cell count in BALF.Lung IL-17A protein lev-el and IL-1β,IL-5,IL-17A,Eotaxin,and Mu5ac mRNA expression in lung tissue were elevated,while the protein ex-pression of E-CDH in airway epithelium and the mRNA expression of Cdh1 in the lung tissues were decreased.The pro-tein expression of ARG-1 around the airway and the mRNA level of Arg-1 in the lung tissues were increased.DEX treat-ment partially improved airway inflammation,excessive mucus secretion,and cytokine levels induced by ST combined with LPS,but DEX had no significant effect on the expression of Cdhl,IL-5,and IL-1β in lung tissue.In conclusion,the model in-duced by ST plus LPS might be an animal model of the advanced stage from eosinophil asthma to neutrophilic asthma.
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