Role of Fuzi Lizhong pill anti-inflammatory and antidiarrheal effects on rats with irinotecan-induced diarrheal based on NLRP-3 inflammasomes
To investigate the anti-inflammatory and antidiarrheal effects of Fuzi Lizhong pill and to study its mecha-nisms through NLRP-3 inflammasomes.Wistar rats were divided into control group,model group,berberine group,IKK-β inhibitor group,Fuzi Lizhong pill low,middle and high dose groups(10,20,40 g·kg-1 respectively).A rat model of irinotecan-induced diarrhea was established in the model group,berberine group,IKK-β inhibitor group,Fuzi Lizhong pill low,middle and high dose groups.After successful modeling,except for the model group,each group was given the corresponding drug for gastric gavage,and the control group was given distilled water of equal volume,and the gastric gavage was continuously gavaged for 30 days and then to detect relevant indexes.The results showed that in the model group the histopathological changes of the colon in the model group and its ultrastructural damage were serious,moreover,the levels of IL-1β,IL-18 and TNF-a,the relative expressions of mRNA of NLRP-3,Caspase-1,ASC,GSDMD and the relative expressions of NLRP-3,Caspase-1 and ASC proteins all significantly increased compared to the control group.However,berberine group,IKK-β inhibitor group,Fuzi Lizhong pill low,middle and high dose groups can im-prove above indexes in different degrees compared to the model group.It is supposed that Fuzi Lizhong pill can alleviate inflammation and diarrhea by inhibiting the activation of NLRP-3 inflammasomes.
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