Construction of two-component system bfmR gene deletion strain of Acinetobacter baumannii and analysis of its biological characteristics
To study the effect of two-component system bfmR gene on the biological characteristics of Acinetobacter baumannii,the bfmR gene deletion mutant of A.baumannii strain AB43 was constructed using RecA homologous recom-bination and the changes of biological characteristics were observed.The bfmR gene was analyzed by ExPASY-Prot-Param,SMART,SOPMA and SWISS-MODEL.The results showed that the growth rate of bfmR gene deletion mutant(AB43△bfmR)was significantly reduced,the autopolymerization ability and intracellular superoxide dismutase(SOD)ac-tivity of the bacteria were significantly decreased,the biofilm forming ability,adhesion and invasion ability were not sig-nificantly changed,and the sensitivity to ceftriaxone was significantly increased.The bioinformatics analysis results showed that the bfmR protein was a relatively stable hydrophilic protein,alpha-helix was the highest in the secondary structure,accounting for 36.13％of the total.The bfmR gene of A.baumannii strain AB43 was closely related to that of the standard strain ATCC 17978 and ATCC 19606.In conclusion,the construction of A.baumannii AB43△bfmR provides an experimental basis for further exploration of the regulatory mechanism of bfmR gene in the two-component system.
国家科技期刊平台
NSTL
万方数据
