LGALS1通过PI3K/Akt信号通路促进胃癌血管生成的研究
LGALS1 promotes angiogenesis of gastric cancer through PI3K/Akt signaling pathway
仇必军 1崇杨2
作者信息
- 1. 扬州大学附属医院,江苏扬州 225009;扬州大学附属江都人民医院,江苏扬州 225100
- 2. 扬州大学附属医院,江苏扬州 225009
- 折叠
摘要
通过生物信息学和免疫组织化学分析半乳糖凝集素1(lectin galactoside-binding soluble 1,LGALS1)基因对胃癌患者预后以及对胃癌血管生成指标的影响,并构建不同表达水平LGALS1胃癌细胞系,通过Transwell、小管形成、qRT-PCR及Western blot研究LGALS1在胃癌血管生成中的作用机制.结果表明:LGALS1表达与胃癌患者总生存期呈正相关,有关血管生成通路中PI3K/Akt通路与LGALS1基因相关,胃癌组织中LGALS1表达水平与CD31表达呈正相关;胃癌细胞中LGALS1表达能明显促进内皮细胞增殖及血管形成能力,且通过调控p-Akt表达促进内皮细胞的迁移能力.综上,LGALS1诱导肿瘤血管新生进而促进胃癌的进展和转移,其原因可能与激活PI3K/Akt信号通路有关.
Abstract
To investigate the mechanism of lectin galactoside-binding soluble 1(LGALS1)gene in the angiogenesis of gastric cancer.The effects of LGALS1 on prognosis and angiogenesis in gastric cancer were analyzed by bioinformatics and immunohistochemistry.Different expression LGALS1 gastric cancer cell lines were constructed.The mechanism of LGALS1 in gastric cancer angiogenesis was studied by Transwell,tubule formation,qRT-PCR and Western blot.The re-sults showed that the expression of LGALS1 was directly proportional to the overall survival of gastric cancer patients.There was a correlation between the PI3K/Akt pathway and LGALS1 gene in the angiogenesis pathway.The expression of LGALS1 in gastric cancer was positively correlated with the expression of CD31.The expression of LGALS1 in gastric cancer cells significantly promoted the proliferation and duct formation of endothelial cells.These promoted the migration of endothelial cells by regulating the expression of p-Akt.In conclusion,LGALS1 gene can promote angiogenesis and tumor progression of gastric cancer,which may be related to the activation of PI3K/Akt signaling pathway.
关键词
胃癌/LGALS1/PI3K/Akt信号通路/血管生成/HUVEC细胞Key words
gastric cancer/LGALS1/PI3K/Akt signaling pathway/angiogenesis/HUVEC cells引用本文复制引用
基金项目
国家自然科学基金资助项目(81802792)
出版年
2024
国家科技期刊平台
NSTL
万方数据