Study on the chemical constituents in the mycelium of endophyte Alternaria sp.QDFB-2 from Ficus carica L.
In order to investigate the chemical constituents in the mycelium of Alternaria sp.QDFB-2,an endophytic fungus isolated from the branch of Ficuscarica L.,isolation and purification of the crude extract from the mycelium of Alternaria sp.QDFB-2 was carried out by silica gel column chromatography,Sephadex LH-20 gel column chromatogra-phy,reversed phase column chromatography and high-performance liquid chromatography.The structures of obtained compounds were then determined by modern spectroscopic techniques such as nuclear magnetic resonance spectroscopy(NMR)and mass spectrometry(MS)as well as by comparison with literatures.The in vitro cytotoxicity of the com-pounds was evaluated by MTT method.Seven compounds were obtained from the crude extract of the mycelium from Al-ternaria sp.QDFB-2,including alternariol(1),tentoxin(2),dihydrotentoxin(3),cyclo(Gly-Pro)(4),cyclo(4-hy-droxy-Pro-Pro)(5),cyclo(S-Leu-S-N-methyl-Phe)(6),and uridine(7).The in vitro cytotoxicity evaluation showed that compounds 1 and 7 could inhibit the proliferation of SMMC-7721 cells with IC50 values of 50.47 μg·mL-1(195.62 μmol·L-1)and 42.50 μg·mL-1(174.18 μmol·L-1),respectively.Compound 1 exhibited moderate prolifer-ation inhibitory activity against A549 cells with an inhibition rate of 52.12％at the concentration of 100 μg·mL-1,while the IC50 values of the positive control cisplatin were 3.46 μg·mL-1(11.53 μmol·L-1)and 5.12 μg·mL-1(17.06 μmol·L-1),respectively.The mycelium of Alternaria sp.QDFB-2 contains rich chemical substances,which is worthy of further re-search.
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