Mining of NaCl stress-related genes in Salix linearistipularis based on FOX hunting system technology
In order to explore the genes related to NaCl stress in Salix linearistipularis,more than 400,000 yeast monoclones of S.linearistipularis cDNAs were obtained based on the FOX hunting system technology using Saccharomy-ces cerevisiae InVscI as material in this study.The transgenic yeast was treated with NaCl stress and screened to obtain 105 resistant yeasts,then they were carried out resistance test,in NaCl stress medium,from which three were signifi-cantly resistant to NaCl compared to the control.Bioinformatics analysis showed that the cDNA of S.linearistipularis transformed from yeast 3 is a full-length cDNA sequence,including all open reading frames,with a coding region of 318 bp,encoding 106 amino acid residues,containing the DABB structural domain,and having very high similarity to DABB-like proteins of other plants.Further results of resistance analysis of this yeast in media with different abiotic stress treatments showed significant resistance to all tested abiotic stresses.This study provides a experimental basis for further understanding of the molecular mechanisms of salt tolerance in woody plants.
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