Therapeutic effects and mechanism of a self-formulated Chinese herbal formula for psoriasis induced by imiquimod in mice
Objective To observe the therapeutic effect of a self-formulated formula for psoriasis on imiquimod induced BALB/c mouse psoriasis model and explore its mechanism of action.Methods The BALB/c mice were randomly divided into the normal group,model group,and oral external group(all n=10).The BALB/c mouse psoriasis like model was induced by using back application of 5%imiquimod cream to verify the therapeutic effect of the self-formulated formula for psoriasis.Before induction with imiquimod cream,as well as on the 3rd,7th,10th,and 12th day after induction,the distribution and tortuous changes of blood vessels in the skin of mice were observed by using photoacoustic imaging technology to evaluate the effect of the formula on abnormal angiogenesis.The local pathological changes in the skin of experimental mice wasevaluated by using the Hematoxylin-Eosin staining.The enzyme linked immunosorbent assay was used to detect changes in the content of cytokines such as tumor necrosis factor(TNF-α),interleukin-17(IL-17),and interleukin-6(IL-6)in mouse skin tissue and serum.By using the flow cytometry,the levels of cytokines such as Treg,Th1,and Th17 in mouse spleen were investigated.Results Compared with the model group,the area and severity index score of psoriasis(PASI)in the oral external group was significantly decreased(P＜0.01),and the levels of TNF-α,IL-17,and IL-6 in the skin of the oral external group were significantly down regulated(P＜0.01).Compared with the model group,the proportion of Treg,Th17,and Th1 cells in the oral external group was significantly reduced(P＜0.05),and the pathological scores of skin tissue(Baker's method)in the oral external group were significantly reduced(P＜0.05).Conclusion Self-formulated formula for psoriasis has a good therapeutic effect on psoriasis and can suppress psoriasis skin lesions through immunosuppressive effects.
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