干细胞外泌体对肺型氧中毒的保护作用和机制
Protective effect and mechanism of mesenchymal stem cell derived exosomes against pulmonary oxygen toxicity
徐胜 1崔立昆 2王舒 3陈杰 2包晓辰 3王越 4赵云鹏4
作者信息
- 1. 海军军医大学转化医学中心干细胞与再生医学研究室,上海 200433;海军军医大学免疫与炎症全国重点实验室,上海 200433;上海市干细胞临床转化研究院,上海 200092
- 2. 海军军医大学免疫与炎症全国重点实验室,上海 200433
- 3. 海军特色医学中心潜水教研室,上海 200433
- 4. 海军军医大学转化医学中心干细胞与再生医学研究室,上海 200433;上海市干细胞临床转化研究院,上海 200092;上海市细胞工程重点实验室,上海 200433
- 折叠
摘要
目的 探讨干细胞外泌体在肺型氧中毒中的保护作用及机制.方法 实验小鼠分为常压空气对照组、高压氧暴露组和高压氧外泌体干预组.在暴露前1 d给予磷酸盐缓冲液(PBS)和外泌体处理,0.23 MPa纯氧暴露8h,分析肺部湿干比、炎症、渗出情况,病理切片分析肺损伤,并检测肺部细胞死亡、抗氧化相关分子表达.结果 干细胞外泌体预处理可明显减轻高压氧暴露引起的肺部损伤,降低湿干比,减少炎症因子分泌和肺部细胞凋亡.结论 干细胞外泌体预防性给药可通过减少细胞死亡和炎症,减轻高压氧引起的肺型氧中毒.
Abstract
Objective To investigate the role and mechanism of exosomes derived from mesenchymal stem cells in hyperbaric oxygen caused pulmonary oxygen toxicity.Methods Mice were divided into the control group that was exposed to normal air,and hyperbaric oxygen exposure groups treated with phosphate buffer saline(PBS)or exosomes,respectively.PBS and exosome treatment were given one day prior to exposure.Mice were subjected to 0.23 MPa pure oxygen for 8 hours.The lung wet-dry ratio,inflammation,exudation and pathological injury were analyzed,while cell death and antioxidant related molecules were detected.Results Pretreatment with exosome significantly attenuated lung injury caused by hyperbaric oxygen exposure by decreasing the lung wet-dry ratio,inflammation,and cell apoptosis.Conclusion Prophylactic administration of exosomes derived from mesenchymal stem cells can alleviate hyperbaric oxygen induced pulmonary oxygen poisoning by attenuating cell death and inflammation.
关键词
外泌体/肺型氧中毒/肺损伤Key words
exosome/pulmonary oxygen toxicity/lung injury引用本文复制引用
基金项目
国家自然科学基金(82071789)
上海市高峰学科建设项目(Ⅳ类)()
出版年
2024
国家科技期刊平台
NSTL
万方数据