CRISPR-Cas12a在食源性致病菌检测中的应用研究进展
Advances in CRISPR-Cas12a in detection of foodborne pathogenic bacteria
兰紫舒 1陈天姣 1李君文 1金敏 1杨栋1
作者信息
- 1. 军事科学院军事医学研究院,天津 300050
- 折叠
摘要
食源性致病菌是影响食品安全的重要因素,部队官兵在军事行动和日常军事生活中也面临着感染风险,迫切需要高效灵敏的快速检测技术.近年随着规律成簇间隔短回文重复序列(CRISPR)技术的发展,具有反式切割活性的CRISPR-Cas体系越来越受到关注.该文系统阐述了基于CRISPR-Cas12a检测技术的基本原理和常规信号读出技术,对其在食源性致病菌检测中的应用现状和发展趋势进行了综述,讨论了面临挑战和未来发展趋势.
Abstract
Foodborne pathogens make much difference to food safety,and troops are also at risk of infection during military operations and daily lives,so there is an urgent need for highly sensitive and rapid detection technologies.In recent years,with the continuous development of clustered regularly interspaced short palindromic repeats(CRISPR)technology,the CRISPR-Cas system with trans-cutting activity has received increasing attention.This paper outlines the basic principles of CRISPR-Cas12a-based detection technology and conventional signal readout technologies.It also analyzes the current status and developmentsof CRISPR-Cas12a in detecting foodborne pathogenic bacteria.The challenges to and prospects of this technology are also described.
关键词
CRISPR-Cas12a/食源性致病菌/水和食品安全/现场检测Key words
CRISPR-Cas12a/foodborne pathogenic bacteria/water and food security/on-site testing引用本文复制引用
基金项目
国家重点研发计划重点专项(2023YFC3205800)
出版年
2024
国家科技期刊平台
NSTL
万方数据