Effects of simulated solar particle events on mouse bone marrow
Objective To explore the effect of bone marrow injury by simulating the radiation from solar particle events in order to address the radiation limit and assess risks during manned deep space exploration.Methods In line with solar particle events(the main component was protons),BALB/c mice(48 mice per group)were irradiated with 90 MeV protons at the doses of 0,0.1,0.3,0.5,1 and 2 Gy.At 3 and 7 days after irradiation.Routine blood counters were employed to detect peripheral blood changes,Giemsa staining was used to detect the ratio of granulocytes to erythrocytes in bone marrow,and flow cytometry was adopted to detect the proportion of bone marrow stem cells,cell subsets and apoptosis before the dose-response relationship and threshold were analyzed.Results In the dose range of 0.1 to 2 Gy,the number of peripheral blood white blood cells and lymphocytes decreased at 3 and 7 days after irradiation and the ratio of granulocytes to erythrocytes in bone marrow and bone marrow cell subsets were abnormal as the dose increased.Seven days after irradiation,the platelet count decreased.The minimum dose that caused significant changes was 0.5 Gy,13 models with dose-response relationships were obtained,and the minimum values of ED25,ED50 and ED63 were 0.25,0.58 and 0.76 Gy,respectively.Conclusion A total of 13 dose-response relationship models of proton-induced bone marrow injury in mice have been obtained,and the dose threshold of proton-induced bone marrow injury ranges from 0.25 to 0.76 Gy.
deep space radiationprotonsmicebone marrow injuredose effect relationshipthreshold
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