可负载细胞的注射用复合涂层PLGA多孔支架制备及评价
Preparation and evaluation of injectable PLGA porous scaffolds with composite coating for cell loading
闫博华 1邵天娇 1刘壮 1崔静宇 1高静 1高翔1
作者信息
- 1. 军事科学院军事医学研究院,北京 100850
- 折叠
摘要
目的 制备聚多巴胺(PDA)和羧甲基壳聚糖(CMC)复合涂层的注射用乳酸-羟基乙酸共聚物(PLGA)多孔支架,评估其生物相容性,探索其负载细胞用于组织工程的潜力.方法 利用W/O/W复乳乳化溶剂挥发法制备注射用PLGA多孔微粒,随后通过PDA和CMC先后涂层制得多孔支架.以电子扫描显微镜表征支架形貌,ImageJ软件测定粒径和孔径分布.通过细胞与支架共孵育进行细胞毒性评估,利用CCK-8检测细胞活性.扫描电子显微镜和DAPI染色后荧光显微镜观察支架负载细胞的形态.结果 制备的PLGA-PDA-CMC多孔支架平均粒径为(313.69±4.91)µm,平均孔径为(28.99±0.74)μm.扫描电子能谱显示PDA涂层成功,X射线光电子能谱显示PDA和CMC涂层成功.PDA涂层后,水接触角减小,证明PDA涂层改善了PLGA微粒的亲水性.CCK-8实验表明,PLGA-PDA-CMC 多孔支架具有良好的安全性.扫描电子显微镜和荧光显微镜证实细胞在PLGA-PDA-CMC多孔支架上的黏附.结论 成功制备PLGA-PDA-CMC多孔支架,PDA和CMC涂层改善了PLGA微粒的亲水性并促进细胞黏附,所设计的支架可负载细胞应用于组织工程研究.
Abstract
Objective To prepare injectable poly(lactic-co-glycolic)acid(PLGA)microparticle scaffolds coated with a composite of polydopamine(PDA)and carboxymethyl chitosan(CMC),and to evaluate their biocompatibility and capacity for cell loading in tissue engineering.Methods The injectable PLGA microparticles were prepared using the W/O/W double-emulsion solvent evaporation method,followed by PDA and CMC coatings to prepare the porous scaffolds that were morphologically characterized while the size distribution of particles and pores was determined.Cytotoxicity was assessed via co-incubation of the scaffolds with cells,while cell viability was evaluated using the CCK-8 assay.The morphology of cells loaded onto the scaffolds was examined using scanning electron microscopy and DAPI staining.Results The average particle size of the prepared PLGA-PDA-CMC porous scaffolds was(313.69±4.91)μm,and the average pore size was(28.99±0.74)μm.Scanning electron microscopy confirmed the success of the PDA coating while X-ray photoelectron spectroscopy proved the success of the PDA and CMC coatings.The reduced water contact angle post-PDA coating indicated enhanced hydrophilicity of the PLGA microparticles.CCK-8 assay results demonstrated the safety of the PLGA-PDA-CMC porous scaffolds.Scanning electron microscopy and fluorescence microscopy confirmed cell adhesion on the PLGA-PDA-CMC porous scaffolds.Conclusion The successful preparation of PLGA-PDA-CMC porous scaffolds,featuring PDA and CMC coatings,can enhance the hydrophilicity and cell adhesion properties of PLGA microparticles.These scaffolds can be potentially used in tissue engineering research.
关键词
乳酸-羟基乙酸共聚物/多孔支架/聚多巴胺/羧甲基壳聚糖/组织工程Key words
PLGA/porous scaffold/polydopamine/carboxymethyl chitosan/tissue engineering引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据