Content and Antioxidant Activities of Polysaccharides in 5 Species of the Family of Berberidaceae
The content of polysaccharides in five species of the family of Berberidaceae,including Nandina domestica,Berberis thunbergii,Berberis thunbergii var.atropurpurea,Mahonia fortunei and Mahonia bealei,was determined by phenol-sulfuric acid method,and the antioxidant activities of polysaccharides in these five species were evaluated by ABTS[2,2'-azino-bis (3-ethyl-benzothiazoline6-sulfonic acid) diammonium salt] and DPPH (1,1-diphenyl-2-picrylhydrazyl) assays.The average content of polysaccharides in Nandina domestica,Berberis thunbergii,Berberis thunbergii var.atropurpurea,Mahoniafortunei and Mahonia bealei was 27.82％,14.52％,12.14％,14.44％ and 16.80％,respectively.EC50 values of ABTS' + radical-scavenging capacity were 0.168 8,0.543 6,0.160 4,0.399 1 and 0.168 9 g/L,respectively,and EC50 values of DPPH radical-scavenging capacity were 0.067 8,0.376 9,0.210 4,0.291 7 and 0.222 4 g/L,respectively.There is no significant difference in polysaccharide content among the same genera,while polysaccharide content and antioxidant activities are different among various species.Both ABTS' + and DPPH radical-scavenging capacities were dose-dependent,and the mathematical model based on dose-effect relationship had high goodness of fit.The content and antioxidant activities of polysaccharides in Nandina domestica and Mahonia bealei were relatively high,which shows great potential for exploitation and utilization.
polysaccharideantioxidant activityberberidaceaepurificationmodernization technology of traditional Chinese medicines
NETL
NSTL
万方数据
维普
