首页|栝楼籽多糖的提取工艺、初级结构及降血糖活性

栝楼籽多糖的提取工艺、初级结构及降血糖活性

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为探究栝楼籽多糖的提取工艺、初级结构及降血糖活性,采用热水浸提法对栝楼籽多糖进行提取,经葡聚糖凝胶G-150纯化得到均质多糖(WHP-Ⅰ)后进行了初步表征.通过体外和体内联合法测试了栝楼籽多糖的降血糖活性.得到栝楼籽多糖的最优提取条件为提取温度80℃、提取时间100 min、料液比1∶25(g∶mL)、浸提1次,在上述条件下,WHP-Ⅰ平均提取率为19.01%.WHP-Ⅰ的重均相对分子质量为4.765×104,并含有5种糖残基,WHP-Ⅰ表面较为平整,有孔状结构,且附着有细小的颗粒.WHP-Ⅰ对α-葡萄糖苷酶的半抑制浓度(IC50)为 15.837 g/L,WHP-Ⅰ具有减缓Ⅱ型糖尿病(T2DM)引起脏器损伤的潜力,给药 4 周后,中剂量组M-WHP-Ⅰ[200 mg/(kg·bw)]和高剂量组 H-WHP-Ⅰ[300 mg/(kg·bw)]小鼠的血糖值分别降低了 32.13%和42.27%,且WHP-Ⅰ对糖尿病小鼠的肾脏和肝脏具有修复作用.
Extraction process,primary structure and hypoglycemic activity of polysaccharide from Trichosanthes kirilowii Maxim.seed
In order to optimize its extraction process and investigate its primary structure and hypoglycemic activity,the homogenous polysaccharide(WHP-Ⅰ)was obtained from Trichosanthes Kirilowii Maxim.seed by hot water extraction,purified by glucan gel G-150,preliminarily characterized and analyzed for its hypoglycemic performance both in vitro and in vivo.Under the optimal extraction conditions of temperature 80℃,extraction time 100 min,solid-liquid ratio 1∶25(g∶mL),and one time extraction,the average extraction rate of polysaccharide was 19.01%.Results from characterization indicated WHP-Ⅰ had a mass-average relative molecular mass of 4.765×104,contained five sugar residues,and displayed a flat surface as well as a porous structure with fine particles attached.Hypoglycemic activity analysis showed that WHP-Ⅰ,with a half-inhibitory concentration(IC50)against α-glucosidase of 15.837 g/L,reduced the blood glucose level by 32.13%with a dose of 200 mg/(kg·bw)and by 42.27%with a dose of 300 mg/(kg·bw)after 4 weeks of administration,indicating its potential to slow the organ damage caused by diabetes mellitus type Ⅱ(T2DM).Moreover,WHP-Ⅰ also demonstrated capacity of repairing the kidney and liver of diabetic mice.

Trichosanthes kirilowii Maxim.polysaccharidesprocess optimizationstructural characterizationhypoglycemic activitymodernization technology of traditional Chinese medicines

宋巧英、张坤朋、翁少亭、李淑艳、宿珍、孔令琦

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安阳工学院 生物与食品工程学院,河南 安阳 455000

栝楼 多糖 工艺优化 结构表征 降血糖活性 中药现代化技术

安阳工学院博士启动基金安阳市科技重大专项安阳工学院博士后启动基金

BSJ20210262022A02NY002BHJ2022010

2024

10.13550/j.jxhg.20230487

精细化工
大连化工研究院设计院 中国化工学会精细化工专业委员会 辽宁省化工研究院

精细化工

CSTPCD北大核心
影响因子：0.557
ISSN：1003-5214
年,卷(期)：2024.41(1)
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