栝楼籽多糖的提取工艺、初级结构及降血糖活性
Extraction process,primary structure and hypoglycemic activity of polysaccharide from Trichosanthes kirilowii Maxim.seed
宋巧英 1张坤朋 1翁少亭 1李淑艳 1宿珍 1孔令琦1
作者信息
- 1. 安阳工学院 生物与食品工程学院,河南 安阳 455000
- 折叠
摘要
为探究栝楼籽多糖的提取工艺、初级结构及降血糖活性,采用热水浸提法对栝楼籽多糖进行提取,经葡聚糖凝胶G-150纯化得到均质多糖(WHP-Ⅰ)后进行了初步表征.通过体外和体内联合法测试了栝楼籽多糖的降血糖活性.得到栝楼籽多糖的最优提取条件为提取温度80℃、提取时间100 min、料液比1∶25(g∶mL)、浸提1次,在上述条件下,WHP-Ⅰ平均提取率为19.01%.WHP-Ⅰ的重均相对分子质量为4.765×104,并含有5种糖残基,WHP-Ⅰ表面较为平整,有孔状结构,且附着有细小的颗粒.WHP-Ⅰ对α-葡萄糖苷酶的半抑制浓度(IC50)为 15.837 g/L,WHP-Ⅰ具有减缓Ⅱ型糖尿病(T2DM)引起脏器损伤的潜力,给药 4 周后,中剂量组M-WHP-Ⅰ[200 mg/(kg·bw)]和高剂量组 H-WHP-Ⅰ[300 mg/(kg·bw)]小鼠的血糖值分别降低了 32.13%和42.27%,且WHP-Ⅰ对糖尿病小鼠的肾脏和肝脏具有修复作用.
Abstract
In order to optimize its extraction process and investigate its primary structure and hypoglycemic activity,the homogenous polysaccharide(WHP-Ⅰ)was obtained from Trichosanthes Kirilowii Maxim.seed by hot water extraction,purified by glucan gel G-150,preliminarily characterized and analyzed for its hypoglycemic performance both in vitro and in vivo.Under the optimal extraction conditions of temperature 80℃,extraction time 100 min,solid-liquid ratio 1∶25(g∶mL),and one time extraction,the average extraction rate of polysaccharide was 19.01%.Results from characterization indicated WHP-Ⅰ had a mass-average relative molecular mass of 4.765×104,contained five sugar residues,and displayed a flat surface as well as a porous structure with fine particles attached.Hypoglycemic activity analysis showed that WHP-Ⅰ,with a half-inhibitory concentration(IC50)against α-glucosidase of 15.837 g/L,reduced the blood glucose level by 32.13%with a dose of 200 mg/(kg·bw)and by 42.27%with a dose of 300 mg/(kg·bw)after 4 weeks of administration,indicating its potential to slow the organ damage caused by diabetes mellitus type Ⅱ(T2DM).Moreover,WHP-Ⅰ also demonstrated capacity of repairing the kidney and liver of diabetic mice.
关键词
栝楼/多糖/工艺优化/结构表征/降血糖活性/中药现代化技术Key words
Trichosanthes kirilowii Maxim./polysaccharides/process optimization/structural characterization/hypoglycemic activity/modernization technology of traditional Chinese medicines引用本文复制引用
基金项目
安阳工学院博士启动基金(BSJ2021026)
安阳市科技重大专项(2022A02NY002)
安阳工学院博士后启动基金(BHJ2022010)
出版年
2024
NETL
NSTL
维普
万方数据