沙棘叶提取物的体外抗氧化及乙酰胆碱酯酶抑制能力
In vitro antioxidant activities and acetylcholinesterase inhibition ability of Hippophae rhamnoides L.leaves extract
黄瑜 1张锡宇 1赵海桃 1石统帅 1邱隽蒙 1符群2
作者信息
- 1. 东北林业大学 生命科学学院,黑龙江 哈尔滨 150040
- 2. 东北林业大学 生命科学学院,黑龙江 哈尔滨 150040;黑龙江省森林食品资源利用重点实验室,黑龙江 哈尔滨 150040
- 折叠
摘要
以 1,1-二苯基-2-三硝基苯肼自由基(DPPH·)清除能力、总还原力为指标,评估了沙棘叶提取物的体外抗氧化活性,考察其对乙酰胆碱酯酶的抑制能力.结果表明:5种不同乙醇体积分数的(40%、50%、60%、70%、80%)沙棘叶提取物均有较好的抗氧化活性及乙酰胆碱酯酶抑制能力.以体积分数为 60%乙醇水溶液提取的沙棘叶提取物的 DPPH·清除率、总还原力及乙酰胆碱酯酶抑制能力最强,分别为 85.80%±1.39%,2.45±0.18,97.14%±0.81%,可作为抗氧化剂与乙酰胆碱酯酶抑制剂.该沙棘叶提取物对乙酰胆碱酯酶有较强的抑制能力,半数抑制浓度(IC50)为(1.086±0.144)g/L,对乙酰胆碱酯酶的抑制类型是竞争性大于非竞争性的混合可逆抑制.Auto Dock分子对接及剂效相关性分析表明,沙棘叶活性成分与乙酰胆碱酯酶具有一定的对接亲和力,沙棘叶提取物的抗氧化、酶抑制活性与功能成分之间均存在良好的正相关性(P<0.05),沙棘叶提取物中起主要抗氧化与酶抑制作用的成分为多酚类化合物.
Abstract
Hippophae rhamnoides L.leaves extract was evaluated for its in vitro antioxidant activity using 1,1-diphenyl-2-picrylhydrazyl radical(DPPH·)scavenging capacity and total reducing power as indicators,and further assessed for its acetylcholinesterase inhibition ability.The results showed that all Hippophae rhamnoides L.leaves extracts with ethanol volume fractions of 40%,50%,60%,70%,and 80%showed good antioxidant activity and acetylcholinesterase inhibition ability,while extract with ethanol volume fractions of 60%exhibited the highest DPPH·scavenging rates,total reducing power,and acetylcholinesterase inhibition ability of 85.80%±1.39%,2.45±0.18,and 97.14%±0.81%respectively,indicating its potential as antioxidant and acetylcholinesterase inhibitor.Moreover,this extract displayed a half acetylcholinesterase inhibitory concentration(IC50)of(1.086±0.144)g/L and a mixed reversible inhibition mechanism against acetylcholinesterase based on the kinetic analysis of enzyme inhibition that was more competitive than non-competitive.Data from Auto Dock molecular docking and dose-effect correlation analysis indicated that the active ingredients of Hippophae rhamnoides L.leaves had a certain docking affinity with acetylcholinesterase.The antioxidant and enzyme inhibitory activities of the extract were positively correlated with the functional ingredients polyphenols(P<0.05).
关键词
沙棘叶/活性成分/乙酰胆碱酯酶/体外抗氧化/分子对接/中药现代化技术Key words
Hippophae rhamnoides L.leaves/active ingredients/acetylcholinesterase/in vitro antioxidation/molecular docking/modernization technology of traditional Chinese medicines引用本文复制引用
基金项目
"十三五"国家重点研发计划项目(2016YFC0500307-07)
出版年
2024
NETL
NSTL
维普
万方数据