In vitro antioxidant activities and acetylcholinesterase inhibition ability of Hippophae rhamnoides L.leaves extract
Hippophae rhamnoides L.leaves extract was evaluated for its in vitro antioxidant activity using 1,1-diphenyl-2-picrylhydrazyl radical(DPPH·)scavenging capacity and total reducing power as indicators,and further assessed for its acetylcholinesterase inhibition ability.The results showed that all Hippophae rhamnoides L.leaves extracts with ethanol volume fractions of 40%,50%,60%,70%,and 80%showed good antioxidant activity and acetylcholinesterase inhibition ability,while extract with ethanol volume fractions of 60%exhibited the highest DPPH·scavenging rates,total reducing power,and acetylcholinesterase inhibition ability of 85.80%±1.39%,2.45±0.18,and 97.14%±0.81%respectively,indicating its potential as antioxidant and acetylcholinesterase inhibitor.Moreover,this extract displayed a half acetylcholinesterase inhibitory concentration(IC50)of(1.086±0.144)g/L and a mixed reversible inhibition mechanism against acetylcholinesterase based on the kinetic analysis of enzyme inhibition that was more competitive than non-competitive.Data from Auto Dock molecular docking and dose-effect correlation analysis indicated that the active ingredients of Hippophae rhamnoides L.leaves had a certain docking affinity with acetylcholinesterase.The antioxidant and enzyme inhibitory activities of the extract were positively correlated with the functional ingredients polyphenols(P<0.05).
Hippophae rhamnoides L.leavesactive ingredientsacetylcholinesterasein vitro antioxidationmolecular dockingmodernization technology of traditional Chinese medicines
NETL
NSTL
万方数据
维普
