季铵盐化糖原衍生物/明胶复合水凝胶抗菌材料的制备及性能
Preparation and properties of quaternary ammonium glycogen derivative-gelatin composite hydrogel as antibacterial material
纪倩 1徐晓宇 1陈曦 1陈敬华1
作者信息
- 1. 江南大学 生命科学与健康工程学院,江苏 无锡 214122
- 折叠
摘要
首先,利用乙二胺对糖原进行氨基化修饰得到氨基化糖原衍生物(N-Gly);接着,利用2,3-环氧丙基三甲基氯化铵(简称季铵盐)对N-Gly进行部分季铵盐化修饰,得到季铵盐化糖原衍生物(QA-N-Gly);然后,以明胶为基材通过酰胺键交联制备季铵盐化糖原衍生物/明胶复合水凝胶(QA-N-Gly/Gel).对糖原衍生物进行FTIR、1HNMR、粒径、Zeta电位以及微观形貌的考察,并对QA-N-Gly/Gel水凝胶进行了理化性能和生物性能评价.结果表明,糖原的氨基化和季铵盐化修饰成功,QA-N-Gly为均匀分散的纳米粒子,粒径分布在100～200 nm,Zeta电位为(93.9±1.7)mV,粒子带有明显的正电荷,具有抗菌性.糖原衍生物的加入可以提高明胶基水凝胶体系的机械强度和稳定性,QA-N-Gly/Gel的断裂应力为66.8 kPa,压缩模量为13.3 kPa.QA-N-Gly/Gel具有明显的抑菌作用,对金黄色葡萄球菌和大肠杆菌的抑菌率分别达到97%和 50%.此外,QA-N-Gly/Gel无溶血性,细胞毒性低,NIH-3T3细胞的48 h存活率在80%以上.
Abstract
Aminated glycogen derivative(N-Gly)was firstly prepared from glycogen by amination with ethylenediamine,and then partially modified by 2,3-epoxypropyl trimethyl ammonium chloride(quaternary ammonium salt)to obtain cationic quaternary ammonium glycogen derivative(QA-N-Gly),which was further crosslinked with gelatin via amide bonding to synthesize quaternary ammonium glycogen derivative-gelatin composite hydrogel(QA-N-Gly/Gel).The glycogen derivatives obtained were characterized by FTIR and 1HNMR,and analyzed for particle size distribution,Zeta potential and morphology.Meanwhile,QA-N-Gly/Gel hydrogel was further evaluated for analyses on physicochemical and biological properties.The results showed that glycogen was successfully aminated and quaternary ammonium modified.The QA-N-Gly,uniformly dispersed nanoparticles with a size distribution of 100～200 nm and a Zeta potential of(93.9±1.7)mV exhibited distinctly positive charges and antibacterial activity.QA-N-Gly/Gel displayed a fracture stress of 66.8 kPa and compression modulus of 13.3 kPa,indicating improved mechanical strength and stability due to addition of glycogen derivative.QA-N-Gly/Gel also showed obvious antibacterial effect on Staphylococcus aureus and Escherichia coli,with the corresponding inhibition rate reaching 97%and 50%,respectively.In addition,QA-N-Gly/Gel showed no hemolysis and low cytotoxicity,with the 48 h survival rate of NIH-3T3 cells>80%.
关键词
糖原/季铵盐/明胶/水凝胶/抗菌材料/功能材料Key words
glycogen/quaternary ammonium salt/gelatin/hydrogels/antibacterial materials/functional materials引用本文复制引用
出版年
2024
NETL
NSTL
万方数据