Preparation and properties of quaternary ammonium glycogen derivative-gelatin composite hydrogel as antibacterial material
Aminated glycogen derivative(N-Gly)was firstly prepared from glycogen by amination with ethylenediamine,and then partially modified by 2,3-epoxypropyl trimethyl ammonium chloride(quaternary ammonium salt)to obtain cationic quaternary ammonium glycogen derivative(QA-N-Gly),which was further crosslinked with gelatin via amide bonding to synthesize quaternary ammonium glycogen derivative-gelatin composite hydrogel(QA-N-Gly/Gel).The glycogen derivatives obtained were characterized by FTIR and 1HNMR,and analyzed for particle size distribution,Zeta potential and morphology.Meanwhile,QA-N-Gly/Gel hydrogel was further evaluated for analyses on physicochemical and biological properties.The results showed that glycogen was successfully aminated and quaternary ammonium modified.The QA-N-Gly,uniformly dispersed nanoparticles with a size distribution of 100～200 nm and a Zeta potential of(93.9±1.7)mV exhibited distinctly positive charges and antibacterial activity.QA-N-Gly/Gel displayed a fracture stress of 66.8 kPa and compression modulus of 13.3 kPa,indicating improved mechanical strength and stability due to addition of glycogen derivative.QA-N-Gly/Gel also showed obvious antibacterial effect on Staphylococcus aureus and Escherichia coli,with the corresponding inhibition rate reaching 97%and 50%,respectively.In addition,QA-N-Gly/Gel showed no hemolysis and low cytotoxicity,with the 48 h survival rate of NIH-3T3 cells>80%.
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