首页|弯萼金丝桃总黄酮提取及抗氧化、降糖活性

弯萼金丝桃总黄酮提取及抗氧化、降糖活性

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通过单因素和响应面实验探究5种提取工艺(超声辅助提取、酸解提取、酶解提取、热水提取、热醇提取)对弯萼金丝桃总黄酮(TF)提取量的影响.采用HPLC测定并分析主要黄酮苷元的分布及含量,并进一步考察了TF与体外抗氧化活性[1,1-二苯基-2-三硝基苯肼自由基(DPPH·)清除能力、2,2'-联氮-双(3-乙基苯并噻唑啉-6-磺酸)二铵盐自由基(ABTS·)清除能力、·OH清除能力、还原能力]的相关性及对α-葡萄糖苷酶的体外降糖能力.结果表明,超声辅助提取TF提取量最高.响应面优化最佳提取工艺为:超声提取温度68 ℃、提取时间23 min、乙醇体积分数24%、溶剂与样品的液料比(mL∶g)63∶1.在该条件下,TF提取量为34.85 mg RT/g[以每克弯萼金丝桃中黄酮类化合物相当于芦丁(RT)的质量表示].经HPLC鉴定出TF中含有7种主要黄酮类化合物,其中,含量最高的为槲皮素-3-O-洋槐糖-7-O-鼠李糖苷和槲皮素,分别为3.897和2.874 mg/g;TF质量浓度与DPPH·清除能力、ABTS·清除能力、·OH清除能力、还原能力呈显著正相关,TF对α-葡萄糖苷酶的降糖能力可达到92.6%.
Total flavonoids from Hypericum benthamii:Extraction,antioxidant and hypoglycemic activity
The influence of five extraction processes(ultrasound-assisted extraction,acid extraction,enzymatic extraction,hot water extraction,hot alcohol extraction)on the total flavonoids(TF)from Hypericum benthamii was investigate by single factor and response surface experiments,with the distribution and content of major flavonoid glycosides determined and analyzed by HPLC.The correlation between TF and in vitro antioxidant activity[1,1-diphenyl-2-trinitrophenylhydrazine free radical(DPPH·)scavenging ability,2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)diammonium salt(ABTS·)scavenging ability,·OH scavenging ability,reducing ability]along with its in vitro hypoglycemic ability to α-glucosidase were further investigated.The results showed that ultrasound-assisted extraction achieved the highest TF content,and the best extraction process optimized by response surface experiment was obtained.Under the conditions of ultrasonic extraction temperature 68 ℃,extraction time 23 min,ethanol volume fraction 24%,liquid to material ratio of solvent to sample(mL∶g)63∶1,the TF content reached 34.85 mg RT/g[Equivalent rutin(RT)mass per gram of TF compound expressed].Seven major flavonoids in TF was identified by HPLC,with the most abundant flavonoid compounds of quercetin-3-O-palmitoyl-7-O-rhamnoside with 3.897 mg/g and quercetin with 2.874 mg/g.There was a significant positive correlation between TF mass concentration and DPPH·scavenging ability,ABTS·scavenging ability,·OH scavenging ability and reducing ability.The hypoglycemic capacity of TF to α-glucosidase could reach 92.6%.

Hypericum benthamiitotal flavonoidsextraction processHPLCantioxidant activityhypoglycemic activitymodernization technology of traditional Chinese medicines

王俊龙、蔺永刚、陈凤霞、时文盼、陈禹

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伊犁师范大学新疆生物质资源清洁转化与高值化利用重点实验室,新疆伊宁 835000

新疆医科大学药学院,新疆乌鲁木齐 830017

弯萼金丝桃 总黄酮 提取工艺 高效液相色谱 抗氧化活性 降糖活性 中药现代化技术

新疆维吾尔自治区自然科学基金伊犁师范大学校级重点项目(2023)伊犁师范大学提升综合实力专项重点项目伊犁师范大学科研创新团队项目

2022D01C4552023YSYY00222XKZZ08CXZK2021003

2024

精细化工
大连化工研究院设计院 中国化工学会精细化工专业委员会 辽宁省化工研究院

精细化工

CSTPCD北大核心
影响因子:0.557
ISSN:1003-5214
年,卷(期):2024.41(5)
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