Microbial fermentation purification of Sapindus mukorossi extract solution and anti-acne effect of Lactobacillus Sapindus fermentation broth
Sapindus mukorossi Gaertn extracts was often added into care products as a natural active raw material.In order to promote the application of Sapindus mukorossi Gaertn extracts in daily chemical industry,the water extract was purified by microbial fermentation method.Sapindus mukorossi fermentation liquid(SF)was then prepared using Lactobacillus plantarum as fermentation strain,which was selected based on polysaccharide mass concentration and saponin mass fraction,and evaluated for its anti-acne performance from the aspects of sebum,inflammation and overgrowth of pathogenic bacteria.The results showed that SF with a mass concentration of 20 μg/mL led to a reduction in the lipid binding rate of SZ95 cells by 26.4％,and NO,tumor necrosis factor-α(TNF-α),interleukin-6(IL-6)and reactive oxygen(ROS)in RAW 264.7 cells induced by lipolyaccharide(LPS)by 52.3％,57.5％,56.2％and 35.9％,respectively.Moreover,the SF exhibited a minimum inhibitory mass concentration,which were measured by double dilution method,against Propionibacterium acnes,Malassezia furfur and Staphylococcus aureus of 3.125,12.5 and 6.25 g/L,respectively.Data from the human efficacy evaluation revealed that the facial acne situation was significantly improved.
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